Effects of sterilisation, humic acid and indigenous microbial formulation on physicochemical properties and macro-micronutrients of dairy farm effluent compost

Edelisa Jenny Radius; Evernessah Eva Dominus; Chong Khim Phin; Syahriel Abdullah; Januarius Gobilik.

Transactions on Science and Technology, 11(3), 147 - 164.

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ABSTRACT
Biostimulant application can enhance compost’s physicochemical properties, nutrient content and performance. Dairy farm effluent compost (DFEC) is a promising planting medium for leafy vegetable production. However, little is known about its quality after biostimulant application. The present study was carried out to evaluate the effects of humic acid (HA) and indigenous microbial formulation (IMF) on DFEC’s physicochemical properties and macro-micronutrients. Sterilised (ST) or non-sterilised (NST) DFECs were added with HA, IMF or NPK 15:15:15 fertiliser, or a combination of them; there were eight amendments (A1-A8). The amended DFECs were re-used four times for Pak Choy cultivation; the plants were planted in forty-eight 18 L pots. DFEC samples were collected from each pot at the second (MR2), third (MR3) and fourth (MR4) harvesting of the Pak Choy, and the physicochemical properties (pH, EC, C/N ratio, OM, OC, WHC) and macro-micronutrients (N, P, K, Mg, Ca, Na, Mn, Fe, Zn and Cu) were evaluated. The macro-micronutrients were analysed using a CHN analyser and ICP-OES. The data were analysed by performing three-way ANOVA at a = 0.05 using SAS. ST-DFEC had higher pH, EC, C/N ratio, WHC, and macro-micronutrients (P, K, Ca, Na, Mn, Fe, and Cu) but lower OM and OC. HA, IMF or HA+IMF application did not significantly affect the DFEC’s physicochemical properties and macro-micronutrients. The effects were significant only after NPK addition (A5-A8). HA+IMF+NPK (A8) was the best amendment to improve P, K, Ca, Na, Mn, Fe, and Cu levels. Nutrient content was better in the second and fourth re-usage of the compost. The data indicated that DFEC’s agronomic quality could be improved by sterilization, NPK+HA+IMF addition or NPK and at least one of the biostimulants, re-usage with NPK and biostimulant applications, or a combination of those amendments.

KEYWORDS: Dairy farm effluent compost; Humic acid; Indigenous microbes; Pak Choy; Compost amendments



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REFERENCES
  1. Aggelides, S. M. & Londra, P. A. 2000. Effects of compost produced from town wastes and sewage sludge on the physical properties of a loamy and a clay soil. Bioresource Technology, 71(3), 253-259.
  2. Al Chami, Z., Cavoski, I., Mondelli, D. & Miano, T. 2013. Effect of compost and manure amendments on zinc soil speciation, plant content, and translocation in an artificially contaminated soil. Environmental Science and Pollution Research International, 20(7), 4766-4776.
  3. Al-Bataina, B. B., Young, T. M. & Ranieri, E. 2016. Effects of compost age on the release of nutrients. International Soil and Water Conservation Research, 4, 230-236.
  4. Alkharabsheh, H. M., Seleiman, M. F., Battaglia, M. L., Shami, A., Jalal, R. S., Alhammad, B. A., Almutairi, K. F. & Al-Saif, A. M. 2021. Biochar and its broad impacts in soil quality and fertility, nutrient leaching and crop productivity: A Review. Agronomy, 11, 993.
  5. Anwar, Z., Irshad, M., Mahmood, Q., Hafeez, F. & Bilal, M. 2017. Nutrient uptake and growth of spinach as affected by cow manure co-composted with poplar leaf litter. International Journal of Recycling of Organic Waste in Agriculture, 6, 79-88.
  6. Bachar, A., Al-Ashhab, A., Soares, M. I. M., Sklarz, M. Y., Angel, R., Ungar, E. D. & Gillor, O. 2010. Soil microbial abundance and diversity along a low precipitation gradient. Microbial Ecology, 60(2), 453-461.
  7. Basri, N. K. M., Gobilik, J., Lum, M. S., Chong, K. P., Ador, K., Jalloh, M. B. & Chin, F. S. 2021. Isolation and characterization of multifunction beneficial bacteria from dairy farm effluent compost. Transactions on Science and Technology, 8(3), 324-330.
  8. Belal, E. E., El Sowfy, D. M. & Rady, M. M. 2019. Integrative soil application of humic acid and sulfur improves saline calcareous soil properties and barley plant performance. Communications in Soil Science and Plant Analysis, 50(15), 1919-1930.
  9. Berendsen, R. L., Pieterse, C. M. & Bakker, P. A. 2012. The rhizosphere microbiome and plant health. Trends Plant Science, 17, 478-486.
  10. Bernal, M. P., Alburquerque, J. A. & Moral, R. 2009. Composting of animal manures and chemical criteria for compost maturity assessment. A review. Bioresource Technology, 100(22), 5444-5453.
  11. Bernard, J. M. 1963. Forest floor moisture capacity of the New Jersey pine barrens. Ecology, 44, 574-576.
  12. Berns, A. E., Philipp, H., Narres, H. D., Burauel, P., Vereecken, H. & Tappe, W. 2008. Effect of gamma-sterilisation and autoclaving on soil organic matter structure as studied by solid state NMR, UV and fluorescence spectroscopy. European Journal of Soil Science, 59(3), 540-550.
  13. Canellas, L. P., Olivares, F. L., Okorokova-Facanha, A. L. & Facanha, A. R. 2002. Humic acids isolated from earthworm compost enhance root elongation, lateral root emergence, and plasma membrane H+-ATPase activity in maize roots. Plant Physiology, 130, 1951-1957.
  14. Chaves, B., De Neve, S., Boeckx, P., Van Cleemput, O. & Hofman, G. 2005. Screening organic biological wastes for their potential to manipulate the N release from N-rich vegetable crop residues in soil. Agriculture, Ecosystems & Environment, 111(1-4), 81-92.
  15. Chefetz, B., Hatcher, P. G., Hadar, Y. & Chen, Y. 1996. Chemical and biological characterization of organic matter during composting of municipal solid waste. Journal of Environmental Quality, 25, 776-785.
  16. Chen, Y., Sun, X., Zhou, B., Gao, Q., Zhang, L. & Mao, L. 2019. Effects of humic acid, green manure, and chemical fertilizers on soil organic carbon mineralization in rice–wheat cropping systems. Journal of Soils and Sediments, 19(7), 3159-3168.
  17. Dhaliwal, S. S., Naresh, R. K., Mandal, A., Walia, M. K., Gupta, R. K., Singh, R. & Dhaliwal, M. K. 2019. Effect of manures and fertilizers on soil physical properties, build-up of macro and micro-nutrients and uptake in soil under different cropping systems: a review. Journal of Plant Nutrition, 42(20), 2873-2900.
  18. Fiorentino, N., Ventorino, V., Woo, S. L., Pepe, O., De Rosa, A., Giola, L., Lombardi, N., Napolitano, M., Colla, G. & Rouphael, Y. 2018. Trichoderma-based biostimulants modulate rhizosphere microbial populations and improve N uptake efficiency, yield, and nutritional quality of leafy vegetables. Frontiers in Plant Science, 9(6), 1-15.
  19. Fitzpatrick, G. E. 1998. Compost and quality assurance. (https://floridaforce.org/wp-content/uploads/2016/12/CompostUseInFlorida.pdf). Last accessed on 15 July 2024.
  20. Font-Palma, C. 2019. Methods for the Treatment of Cattle Manure—A Review. Journal of Carbon Research, 5, 27.
  21. Garcia, A. C., Santos, L. A., de Souza, L. G. A., Tavares, O. C. H., Zonta, E. & Gomes, E. T. M. 2016. Vermicompost humic acids modulate the accumulation and metabolism of ROS in rice plants. Journal of Plant Physiology, 192, 56-63.
  22. Gerke, J. 2022. The central role of soil organic matter in soil fertility and carbon storage. Soil Systems, 6(2), 33.
  23. Grigatti, M., Giorgioni, M. E., Pilotti, S. & Ciavatta, C. 2012. Stability, nitrogen mineralization capacity and agronomic value of compost-based growing media for lettuce cultivation. Journal of Plant Nutrition, 35(5), 704-25.
  24. Holatko, J., Bielska, L., Hammerschmiedt, T., Kucerik, J., Mustafa, A., Radziemska, M., Kintl, A., Baltazar, T., Latal, O. & Brtnicky, M. 2022. Cattle manure fermented with biochar and humic substances improve the crop biomass, microbiological properties and nutrient status of soil. Agronomy, 12, 368.
  25. Ingram, D. L. 2014. Understanding soilless media test results and their implications on nursery and greenhouse crop management. (https://uknowledge.uky.edu/cgi/viewcontent.cgi?article=1161& context=anr_reports). Last accessed on 15 July 2024.
  26. Insam, H. & Bertoldi, M. 2007. Microbiology of the composting process. In: Diaz, L.F., Bertoldi, M. D., Bidlingmaier, W. & Stentiford, E. (Eds.). Waste Management Series – Compost Science and Technology (Vol. 8). Amsterdam: Elsevier.
  27. Isaac, R. A. & Johnson, W. C. 1975. Collaborative study of wet and dry ashing techniques for the elemental analysis of plant tissue by Atomic Absorption Spectrophotometry. Journal of the AOAC, 58(3), 436-440.
  28. Kebreab, E., France, J., Mills, J. A. N., Allison, R. & Dijkstra, J. 2002. A dynamic model of N metabolism in the lactating dairy cow and an assessment of impact of N excretion on the environment. Journal of Animal Science, 80, 248-59
  29. Khaled, H. & Fawy, H. A. 2011. Effect of different levels of humic acids on the nutrient content, plant growth, and soil properties under conditions of salinity. Soil Science and Water Research, 6(1), 21-29.
  30. Lehmann, J. & Kleber, M. 2015. The contentious nature of soil organic matter. Nature, 528(7580), 60-68.
  31. Li, K., DiLegge, M. J., Minas, I. S., Hamm, A., Manter, D. & Vivanco, J. M. 2019a. Soil sterilization leads to re-colonization of a healthier rhizosphere microbiome. Rhizosphere, 12, 100176.
  32. Li, X., Wei, H. & Gao, H. 2023. Influence of humic substances on water retention in sandy soils. Journal of Hydrology, 610, 128140.
  33. Li, Y., Fang, F., Wei, J., Wu, X., Cui, R., Li, G., Zheng, F. & Tan, D. 2019b. Humic acid fertilizer improved soil properties and soil microbial diversity of continuous cropping peanut: a three-year experiment. Scientific Reports, 9, 12014.
  34. Liu, X., Dou, S. & Zheng, S. 2022. Effects of corn straw and biochar returning to fields every other year on the structure of soil humic acid. Sustainability, 14(23), 15946.
  35. Liu, X., Yu, X. & Chen, J. 2021. Enhancing cation exchange capacity of soils through HA applications. Geoderma, 401, 115293.
  36. Makhlof, H. A., Mohammed, H. A. E., Ahmed, G. L. & Emhammed, M. A. 2022. Integrated effect of fertilizers on soil pH, EC and organic matter content. Journal of Pure and Applied Sciences, 21(4), 323-328.
  37. Maludin, A. J., Che Musa, N. A. I., Silip, J. J., Yap, Y. F. & Gobilik, J. 2019. Effects of dairy farm effluent compost on growth and yield of Pak Choy (Brassica rapa L.) in pot system. Transaction on Science and Technology, 6(2), 272-282.
  38. MARDI. 2005. Anggaran Kos Pengeluaran dan Pendapatan untuk Sayuran dan Rempah. Kuala Lumpur: MARDI.
  39. McNamara, N. P., Black, H. I. J., Beresford, N. A. & Parekh, N. R. 2003. Effects of acute gamma irradiation on chemical, physical and biological properties of soils. Applied Soil Ecology, 24, 117-132.
  40. Murad, W., Mustapha, N. H. N. & Siwar, C. 2008. Review of Malaysian agricultural policies with regards to sustainability. American Journal of Environmental Sciences, 4(6), 608-614.
  41. Nannipieri, P., Ascher, J., Ceccherini, M. T., Landi, L., Pietramellara, G. & Renella, G. 2003. Microbial diversity and soil functions. European Journal of Soil Science, 54(4), 655-670.
  42. Ni, H., Zhao, J. & Yang, Z. 2024. Effects of compound fertilizer decrement and water-soluble humic acid fertilizer application on soil properties, bacterial community structure, and shoot yield in Lei Bamboo (Phyllostachys praecox) Plantations in Subtropical China. Forests, 15, 400.
  43. Ochieno, D. M. W. 2022. Soil sterilization eliminates beneficial microbes that provide natural pest suppression ecosystem services against Radopholus similis and Fusarium Oxysporum V5w2 in the endosphere and rhizosphere of tissue culture banana plants. Frontiers in Sustainable Food Systems, 6, 688194.
  44. Okonwu, K. & Mensah, S. I. 2012. Effects of NPK (15:15:15) fertilizer on some growth indices of pumpkin. Asian Journal of Agricultural Research, 6 (3), 137-143.
  45. Pandit, N. R., Thapa, S. & Shrestha, J. 2023. Enhancing compost quality with HA: Implications for nutrient management. Compost Science and Utilization, 31(1), 12-24.
  46. Parmar, P. & Sindhu, S. S. 2013. Potassium solubilisation by rhizosphere bacteria: Influence of nutritional and environmental conditions. Journal of Microbiology Research, 3(1), 25-31.
  47. Parnaudeau, V., Nicolardot, B., Robert, P., Alavoine, G., Pag`es, J. & Duchiron, F. 2006. Organic matter characteristics of food processing industry wastewaters affecting their C and N mineralization in soil incubation. Bioresource Technology, 97(11), 1284-1295.
  48. Phares, C. A. & Akaba, S. 2022. Co-application of compost or inorganic NPK fertilizer with biochar influences soil quality, grain yield and net income of rice. Journal of Integrative Agriculture, 21(12), 3600-3610.
  49. Plaza, C., Fernández, J. M., García-Gil, J. C., Polo, A. & Senesi, N. 2005. Effects of humic substances from organic waste enhancement on nutrient availability and uptake by lettuce. Communications in Soil Science and Plant Analysis, 36(15-16), 2009-2024.
  50. Radius, E. J. 2024. Efficacy of Dairy Farm Effluent Compost Amended with Humic Acid and Indigenous Microbial Formulation on Growth and Yield of Curly Dwarf Pak Choy (Brassica Rapa L.). MSc Thesis, Universiti Malaysia Sabah, Malaysia.
  51. Raheem, S. M., Al-Jaf, H. I. & Tofiq, G. K. 2018. Influence of foliar and soil application of humic acid on growth and yield of lettuce. Euphrates Journal of Agriculture Science, 10(2), 199-204.
  52. Ramesh, A., Sharma, S. K., Sharma, M. P., Yadav, N. & Joshi, O. P. 2014. Plant growth-promoting traits in Enterobacter cloacae subsp. dissolvens MDSR9 isolated from soybean rhizosphere and its impact on growth and nutrition of soybean and wheat upon inoculation. Agricultural Research, 3, 53-66.
  53. Raturi, G., Sharma, Y., Rana, V., Thakral, V., Myaka, B., Salvi, P., Singh, M., Dhar, H. & Deshmukh, R. 2021. Exploration of silicate solubilizing bacteria for sustainable agriculture and silicon biogeochemical cycle. Plant Physiology and Biochemistry, 166, 827–838
  54. Raviv, M. & Lieth, J. H. 2008. Soilless Culture: Theory and Practice (1st edition). Amsterdam: Elsevier.
  55. Razavi, D. S. & Lakzian, A. 2007. Evaluation of chemical and biological consequences of soil sterilisation methods. Caspian Journal of Environmental Sciences, 5(2), 87-91.
  56. SAS. 2016. Statistical Analysis Software (SAS) User’s Guide Version 9.4. Cary: SAS Institute.
  57. Schoebitz, M., Lopez, M. D., Serri, H., Martinez, O. & Zagal, E. 2016. Combined application of microbial consortium and humic substances to improve the growth performance of blueberry seedlings. Journal of Soil Science and Plant Nutrition, 16(4): 1010–1023.
  58. Sible, C. N., Seebauer, J. R. & Below, F. E. 2021. Plant biostimulants: a categorical review, their implications for row crop production, and relation to soil health indicators. Agronomy, 11, 1297.
  59. Singh, S. K., Wu, X., Shao, C. & Zhang, H. 2022. Microbial enhancement of plant nutrient acquisition. Stress Biology, 2 (1): 1-14.
  60. Stowell, R. R. & Bickert, W. G. 1995. Storing and handling sand-laden dairy manure: a description of manageable practices on midwest. (https://archive.lib.msu.edu/DMC/extension_publications/ e2561/E2561-1995.PDF). Last accessed on 15 July 2024.
  61. Sudita, I. D. N., Situmeang, Y. P. & Suarta, M. 2021. Compost and biochar characteristics test of some animal manure waste. International Journal on Advanced Science, Engineering and Information Technology, 11(1), 266-271.
  62. Sun, H., Wei, C., Xu, W., Yang, J., Wang, X. & Qiu, Y. 2019. Characteristics of salt contents in soils under greenhouse conditions in China. Environmental Science and Pollution Research International, 26, 3882-3892.
  63. Tanaka, S., Kobayashi, T., Iwasaki, K., Yamane, S., Maeda, K. & Sakurai, K. 2003. Properties and metabolic diversity of microbial communities in soils treated with steam sterilisation compared with methyl bromide and chloropicrin fumigations. Soil Science and Plant Nutrition, 49(4), 603-610.
  64. Tian, Y., Zhang, X., Liu, J., Chen, Q. & Gao, L. 2009. Microbial properties of rhizosphere soils as affected by rotation, grafting, and soil sterilization in intensive vegetable production systems. Scientia Horticulturae, 123(2), 139-247.
  65. Tiquia, S. M., Richard, T. L. & Honeyman, M. S. 2002. Carbon, nutrient, and mass loss during composting. Nutrient Cycling in Agroecosystems, 62(1), 15-24.
  66. Tiraieyari, N., Hamzah, A. & Samah, B. A. 2014. Organic farming and sustainable agriculture in Malaysia: organic farmers’ challenges towards adoption. Asian Social Science, 10, 4.
  67. Trabelsi, D. & Mhamdi, R. 2013. Microbial inoculants and their impact on soil microbial communities: A review. BioMed Research International, 2013, 863240.
  68. TrendEconomy. 2024. Malaysia/ Imports and Exports/ World/ Cabbages, Cauliflowers, Kohlrabi, Kale/Value (US$) and Value Growth, YoY (%)/ 2011–2022 (https://trendeconomy.com/data/h2/Malaysia/0704). Last accessed 04 February 2024.
  69. Trevors, J. T. 1996. Sterilisation and inhibition of microbial activity in soil. Journal of Microbiological Methods, 26(1-2), 53-59.
  70. Turan, M., Ekinci, M., Kul, R., Kocaman, A., Argin, S., Zhirkova, A. M., Perminova, I. V. & Yildirim, E. 2022. Foliar applications of humic substances together with Fe/Nano Fe to increase the iron content and growth parameters of spinach (Spinacia oleracea L.). Agronomy, 12(9), 2044.
  71. Verma, R., Maurya, B. R., Meena, V. S., Dotaniya, M. L., Deewan, P. & Jajoria, M. 2017. Enhancing production potential of cabbage and improves soil fertility status of indo-gangetic plain through application of bio-organics and mineral fertilizer. International Journal of Current Microbiology and Applied Sciences, 6(3), 301-309.
  72. Walters, S. A. & Midden, K. S. 2018. Sustainability of urban agriculture: vegetable production on green roofs. Agriculture, 8, 168.
  73. Wang, J., Li, Q., Xu, S., Zhao, W., Lei, Y., Song, C. & Huang, Z. 2018. Traits-based integration of multi-species inoculants facilitates shifts of indigenous soil bacterial community. Frontiers in Microbiology, 9(7), 1-13.
  74. Williams, A., Hunter, M. C., Kammerer, M., Kane, D. A., Jordan, N. R., Mortensen, D. A., Smith, R. G., Snapp, S. & Davis, A. S. 2016. Soil water holding capacity mitigates downside risk and volatility in US rainfed maize: time to invest in soil organic matter? PloS One, 11(8), e0160974.
  75. Xie, L., Cakmak, I., Wang, S., Zhang, F. & Guo, S. 2021. Synergistic and antagonistic interactions between potassium and magnesium in higher plants. The Crop Journal, 9(2), 249-256.
  76. Xu, J., Zhang, Y. & Zhao, S. 2022. The effects of HA on soil aggregation and its mechanisms. Soil and Tillage Research, 216, 105262.
  77. Yang, F., Tang, C. & Antonietti, M. 2021. Natural and artificial humic substances to manage minerals, ions, water, and soil microorganisms. Chemical Society Review, 50, 6221–6239.
  78. Zuraihah, I., Aini, Z. & Faridah, M. 2012. Effects of IMO and EM application on soil nutrients, microbial population and crop yield. Journal of Tropical Agriculture and Food Science, 40(2), 257-263.