Effect of Physicochemical Properties of Oil-palm-waste-based Substrates on Mycelia Growth Rate of Pleurotus ostreatus

Aubrey Mei Li Lee; Clament Fui Seung Chin; Jaya Seelan Sathiya Seelan; Huei Hong Lee; Mohd Rashid Mohd Rakib.

Transactions on Science and Technology, 8(3-2), 317 - 323.

Back to main issue

ABSTRACT
Malaysia produces a large quantity of empty fruit bunch (EFB) and oil palm frond (OPF) every year, these wastes are not efficiently utilized, and causing disposal problems and pollute the environment. On the other hand, it has been reported that oyster mushrooms grown on oil palm by-products produced good yield. Thus, this study was carried out to investigate the effect of physical and chemical properties of the substrate on the mycelia growth rate of Pleurotus ostreatus on three treatments: namely, 100% EFB, 100% OPF, and the mixture of 50% EFB and 50% OPF. All treatments had five replicates and arranged in a completely randomized design (CRD). Chemical and physical properties; concentration of C, N, K, P, Cu, Ca, Mg, Fe, Zn, moisture content, ash content, volatile solids content, pH, electrical conductivity, wet bulk density, particle density, and porosity were measured. The number of days taken for mycelia to entirely colonized the substrate bag was recorded. It was found that pH, EC, and the concentration of C, N, P, Cu, Ca, Mg, and Zn were significantly affected by substrate formulations. It took 23-25 days for mycelia to entirely colonized the substrate in all treatments. However, the mycelia growth rate of P. ostreatus was not significantly affected by the physicochemical properties of substrate formulations.

KEYWORDS: Pleurotus ostreatus; mycelia growth rate; empty fruit bunch; oil palm frond; physicochemical properties



Download this PDF file

REFERENCES

  1. Aaron, H. A., Markson, A. A., Bassey, G. A., Agba, M. O., & Akpan, J. B. 2017. Trace element bioaccumulation potentials of Pleurotus ostreatus: its implication in nutrition-based therapy. IOSR Journal of Environmental Science, Toxicology and Food Technology, 11 (4), 55–60. doi: 10.9790/2402-1104015560.
  2. Abdullah, N. & Sulaiman, F. 2013. The Oil Palm Wastes in Malaysia. In: Matovic, M. D. Biomass Now - Sustainable Growth and Use. London: InTechOpen. doi: 10.5772/55302.
  3. Ali, N., Tabi, A. N. M., Zakil, F. A., Fauzi, Q. N. F. M., & Hassan, O. 2013. Yield performance and biological efficiency of empty fruit bunch (EFB) and palm pressed fibre (PPF) as substrates for the cultivation of pleurotus ostreatus. Jurnal Teknologi (Sciences and Engineering), 64(1), 93–99. doi: 10.11113/jt.v64.1243.
  4. Almomany, A. M., Khalaf, N. & Masaed, A. L. 2019. Impact of physical properties of substrate on mycelial growth and yield of two strains of Agaricus bisporus. International conference on Agriculture, Food, Veterinary and Pharmacy Sciences. 16-18 April, 2019. Trabzon, Turkey.
  5. Atri, N. S. & Guleria, L. 2013. Evaluation of vitamin, phytohormone and trace element requirements of Lentinus cladopus lév. International Journal of Pharmacy and Pharmaceutical Sciences, 5 (SUPPL.4), 40 - 42.
  6. Awalludin, M. F., Sulaiman, O., Hashim, R., & Nadhari, W. N. A. W. 2015. An overview of the oil palm industry in Malaysia and its waste utilization through thermochemical conversion, specifically via liquefaction. Renewable and Sustainable Energy Reviews 50, 1469–1484. doi: 10.1016/j.rser.2015.05.085.
  7. Dungani, R., Aditiawati, P., Aprilia, S., Yuniarti, K., Karliati, T., Suwandhi, I., & Sumardi, I. 2018. Biomaterial from Oil Palm Waste: Properties, Characterization and Applications. In: Waisundara, V. Palm Oil. London: InTechOpen. doi: 10.5772/intechopen.76412.
  8. Dzulkefli, N. A. & Zainol, N. 2018. Data on modeling mycelium growth in Pleurotus sp. cultivation by using agricultural wastes via two level factorial analysis. Data in Brief, 20, 1710–1720. doi: 10.1016/j.dib.2018.09.008.
  9. Haimid, M. T. & Rahim, H. 2013. Understanding the mushroom industry and its marketing strategies for fresh produce in Malaysia. Economic and Technology Management Review, 8, 27–37.
  10. Ibekwe, V. I., Azubuike, P. I., Chinakwe, E. C. 2008. Effects of nutrient sources and environmental factors on the cultivation and yield of oyster mushroom (Pleurotus ostreatus). Pakistan Journal of Nutrition, 7(2), 349–351. doi: 10.3923/pjn.2008.349.351.
  11. Isaac, R. A. & Johnson, W. C. 1975. Collaborative study of wet and dry ashing techniques for the elemental analysis of plant tissue by atomic absorption spectrophotometry. Journal of AOAC International, 58(3), 436–440. doi: 10.1093/jaoac/58.3.436.
  12. Kaur, B., & Atri, N. S. 2016. Effect of growth regulators and trace elements on the vegetative growth of Pleurotus sapidus Quél. International Journal of Pharmacy and Pharmaceutical Sciences, 8 (11), 283 - 287.
  13. Kumar, S., Kumar, A., Chand, G., Akhtar, M. N., & Kumar, T. 2018. Optimization of Mycelia Growth Parameters for Pleurotus florida and Pleurotus sajor-caju. International Journal of Current Microbiology and Applied Sciences, 7, 4818–4823.
  14. Mat-Amin, M. Z. and Harun, A. 2015. Competitiveness of the Mushroom Industry in Malaysia. Food and Fertilizer Technology Center Agricultural Policy Articles, 2009, 1–5.
  15. Mbogoh, J. M., Anjichi, V. E., Rotich, F., Ahoya, N. K. 2011. Substrate effects of grain spawn production on mycelium growth of oyster mushroom. Acta Horticulturae, 911, 469–471. doi: 10.17660/ActaHortic.2011.911.54.
  16. Mihilall, Y., Mudhoo, A. & Mohee, R. 2011. Development of a new substrate for the cultivation of the Pleurotus sajor-caju mushroom through controlled composting. Dynamic Soil, Dynamic Plant, 5(2), 82-92.
  17. Owaid, M. N., Abed, I. A. & Al-Saeedi, S. S. S. 2017. Applicable properties of the bio-fertilizer spent mushroom substrate in organic systems as a byproduct from the cultivation of Pleurotus spp. Information Processing in Agriculture, 4(1), 78–82. doi: 10.1016/j.inpa.2017.01.001.
  18. Rakib, M. R. M., Tan, S. Y., Lee, A. M. L. 2020. Corn husk as lignocellulosic agricultural waste for the cultivation of Pleurotus florida mushroom. Bioresources, 15(4), 7980-7991 . doi: 10.15376/biores.15.4.7980-7991.
  19. Rosmiza, M. Z., Davies, W. P., Rosniza Aznie, C. R., Jabil, M. J., & Mazdi, M. 2016. Prospects for increasing commercial mushroom production in Malaysia: challenges and opportunities. Mediterranean Journal of Social Sciences, 7(1), 406-415 doi: 10.5901/mjss.2016.v7n1s1p406.
  20. Sithole, S. C., Mugivhisa, L. L., Amoo, S. O., & Olowoyo, J. O. 2017. Pattern and concentrations of trace metals in mushrooms harvested from trace metal-polluted soils in Pretoria, South Africa. South African Journal of Botany, 108, 315–320. doi: 10.1016/j.sajb.2016.08.010.
  21. Silva, R. M. da, Carmo, C. O. D, Oliveira, T. A. S. D., Figueirêdo, V. R. D., Duarte, E. A. A., & Soares, A. C. F. 2020. Biological efficiency and nutritional value of Pleurotus ostreatus cultivated in agroindustrial wastes of palm oil fruits and cocoa almonds. Arquivos do Instituto Biológico, 87, 1–10. doi: 10.1590/1808-1657000852018.
  22. Singh, P., Hashim, R., Sulaiman, O., & Peng, L. C. 2013. Using biomass residues from oil palm industry as a raw material for pulp and paper industry: Potential benefits and threat to the environment. Environment, Development and Sustainability, 15(2), 367–383. doi: 10.1007/s10668-012-9390-4.
  23. Tang, K. H. D. & Al Qahtani, H. M. S. 2020. Sustainability of oil palm plantations in Malaysia. Environment, Development and Sustainability 22(6), 4999–5023. doi: 10.1007/s10668-019-00458-6.
  24. Tesfay, T., Godifey, T., Mesfin, R. & Kalayu G. 2020. Evaluation of waste paper for cultivation of oyster mushroom (Pleurotus ostreatus) with some added supplementary materials. AMB Expess 10(1), 1-8 doi: 10.1101/694117.
  25. Yadav, B. 2001. Physical and Nutritional factors in relation to growth of Pleurotus sajur-caju (Fr.) Singer. Journal of Phytological Research, 14(1), 95-98.
  26. Yang, W. J., Guo, F. L. & Wan, Z. J. 2013. Yield and size of oyster mushroom grown on rice/wheat straw basal substrate supplemented with cotton seed hull. Saudi Journal of Biological Sciences, 20(4), 333–338. doi: 10.1016/j.sjbs.2013.02.006.
  27. Zakil, F. A., Sueb, M. S. M. & Isha, R. 2019. Growth and yield performance of Pleurotus ostreatus on various agro-industrial wastes in Malaysia. Proceedings of the 2nd International Conference on Biosciences and Medical Engineering (ICBME2019) 2155(1). 6 September, 2019. Bali, Indonesia. pp 020055-1 - 020055-7. doi: 10.1063/1.5125559.