Influence of Ultraviolet-C Radiation on Biochemical Compositions and Genetics of Capsicum Plants

Somashekara Rajashekara; S. S. Khanum; M. Shanthala; K. R. Mallika; Sunita Adaki.

Chilli or Pepper (Capsicum species) is an essential and critical plant shown as vegetable cum spice crop grown for the ornamental, medicinal and pharmaceutical applications. The present paper aimed to understand the relative changes in biochemical composition of the plants because of impacts of the induced mutation and amplified fragment length polymorphism (AFLP) investigation can be utilized to assess the alteration in the DNA structure. Minimum amount of proteins (799.997 µg/ml), carbohydrates (39.13 µg/ml) and free fatty acids (673.2 µg/ml) were found in very high intervening mutated plants. Maximum percentage of inhibition by the peroxidase activity (0.1685 μg/ml), catalase activity (0.078 μg/ml), diphenyl-1-picrylhydrazyl (79.44±1.61 μg/ml) activity and 2, 2’- azino-bis (3-ethylbenzoithioazoline-6-sulphonic acid) (97.02±0.45 μg/ml) activities were found in profoundly exposed mutated plants compared to the normal Capsicum plant species. A dendrogram representing the similarity in DNA bands of Capsicum plants in different time intervals of UV-C radiations showed significant negative affinities. This study encourages us in understanding an induced mutation by UV-radiation on the Chilli plant leaves and its impact on plant cell creation and DNA structure. Therefore, this study brings mutation prompts diverse sorts of basic variations from the norm in cell organelles and its structure of the plant sources.

KEYWORDS: ABTS assay, Catalase activity, DPPH assay, Peroxidase activity, Ultraviolet radiations..



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