Mycolytic Enzyme-Producing Bacteria Demonstrates Antifungal Activities Against Basal Stem Rot Disease Caused by Ganoderma boninense

Wee Shui Shui; Izzatie binti Musa; Kelvin Ling Wen Sin; Peter Morin Nissom.

Transactions on Science and Technology, 8(2), 85 - 90.

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ABSTRACT
The basal stem rot (BSR) disease of oil palm (Elaeis guineensis Jacq.) is caused by the white rot fungus, Ganoderma boninense (G. boninense). This study discusses the use of a biological control approach to treat BSR by using mycolytic enzymes producing bacteria as biocontrol agents against G. boninense. Bacteria producing mycolytic enzymes which degrade fungal cell wall were targeted. The antifungal properties of Acinetobactor calcoaceticus (A. calcoaceticus), Chryseobacterium indologenes (C. indologenes), and Pseudomonas putida (P. putida) were tested against G. boninense. The three strains showed the ability to inhibit the growth of G. boninense in dual culture test, culture filtrate test, double plate assay, and soft agar encapsulation. In dual culture test, all three test strains showed high Percentage Inhibition of Diameter Growth (PIDG) value with P. putida having the highest PIDG value of approximately 90%. As for culture filtrate test, C. indologenes demonstrated the highest PIDG value, approximately 85%. Double plate assay and soft agar encapsulation depicted the similar result for all three test strains which the PIDG value for both tests were 90%. The isolated strains exhibited promising results in anti-Ganoderma testing.

KEYWORDS: Basal stem rot, Ganoderma boninense, mycolytic enzymes, biological control, percentage inhibition of diameter growth (PIDG).



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