Mycolytic Enzyme-Producing Bacteria Demonstrates Antifungal Activities Against Basal Stem Rot Disease Caused by Ganoderma boninense

Wee Shui Shui; Izzatie binti Musa; Kelvin Ling Wen Sin; Peter Morin Nissom.

Transactions on Science and Technology, 8(2), 85 - 90.

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The basal stem rot (BSR) disease of oil palm (Elaeis guineensis Jacq.) is caused by the white rot fungus, Ganoderma boninense (G. boninense). This study discusses the use of a biological control approach to treat BSR by using mycolytic enzymes producing bacteria as biocontrol agents against G. boninense. Bacteria producing mycolytic enzymes which degrade fungal cell wall were targeted. The antifungal properties of Acinetobactor calcoaceticus (A. calcoaceticus), Chryseobacterium indologenes (C. indologenes), and Pseudomonas putida (P. putida) were tested against G. boninense. The three strains showed the ability to inhibit the growth of G. boninense in dual culture test, culture filtrate test, double plate assay, and soft agar encapsulation. In dual culture test, all three test strains showed high Percentage Inhibition of Diameter Growth (PIDG) value with P. putida having the highest PIDG value of approximately 90%. As for culture filtrate test, C. indologenes demonstrated the highest PIDG value, approximately 85%. Double plate assay and soft agar encapsulation depicted the similar result for all three test strains which the PIDG value for both tests were 90%. The isolated strains exhibited promising results in anti-Ganoderma testing.

KEYWORDS: Basal stem rot, Ganoderma boninense, mycolytic enzymes, biological control, percentage inhibition of diameter growth (PIDG).

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  1. Arrebola, E., Sivakumar, D. & Korsten, L. 2010. Effect of Volatile Compounds produced by Bacillus strains on Postharvest Decay in Citrus. Biological Control, 53(1), 122-128.
  2. Azadeh, B. F. & Meon, S. 2009. Molecular Characterization of Pseudomonas aeruginosa UPM P3 from Oil Palm Rhizosphere. American Journal of Applied Sciences, 6(11), 1915-1919.
  3. Davati, N. & Habibi Najafi, M. B. 2013. Overproduction strategies for microbial secondary metabolites: A review. International Journal of Life Science & Pharma Research, 3(1), 23-37.
  4. Effmert, U, Kalderas, J, Warnke, R & Piechulla, B. 2012. Volatile Mediated Interactions between Bacteria and Fungi in the Soil. Journal of Chemical Ecology, 38(6), 665–703.
  5. Elmahdi, S., Kadir, J., Tengku Muda Mohamed, M. Vadamalai, G. & Akter, S. 2015. Isolation, Screening and Characterization of Effective Microbes with Potential for Biological Control of Fusarium wilt of Rock Melon. World Journal of Agricultural Research, 3(1), 11-16.
  6. Fankhauser, D. 1994. Agar Overlay Technique – David Fankhauser ( Last accessed on 7 July 2020.
  7. Fernando, W.G.D., Ramarathnam, R., Krishnamoorthy, A.S. & Savchuk, S.C. 2005. Identification and Use of Potential Bacterial Organic Antifungal Volatiles in Biocontrol. Soil Biology & Biochemistry, 37, 955-964.
  8. Garbeva, P., Hordijk, C., Gerards, S. & Boer, W.D. 2014. Volatiles produced by the Mycophagous Soil Bacterium Collimonas. FEMS Microbiology Ecology, 87(3), 639-649.
  9. Himratul-Aznita, W. H., Mohd-Al-Faisal. N. & Fathilah. A. R. 2011. Determination of the Percentage Inhibition of Diameter Growth (PIDG) of Piper betle Crude Aqueous Extract against Oral Candida species. Journal of Medicinal Plants Research, 5(6), 878–884.
  10. Kiewnick, S. & Sikora, R.A. 2006. Biological Control of the Root- Knot Nematode Meloidogyne incognita by Paecilomyce slilacinus strain 251. Biological Control, 38(2), 179-187.
  11. Köhl, J., Kolnaar, R. & Ravensberg, W. J. 2019. Mode of Action of Microbial Biological Control Agents against Plant Diseases: Relevance beyond Efficacy. Frontiers in Plant Science, 10, 845.
  12. Liu, C.H., Chen, X., Liu, T.T., Lian, B., Gu, Y.C., Caer, V., Xue, Y.R. & Wang, B.T. 2007. Study of the Antifungal Activity of Acinetobacter baumannii LCH001 in vitro and identification of its Antifungal Components. Applied Microbial and Cell Physiology, 76, 459-466.
  13. Maindad, D.V., Kasture, V.M., Chaudhari, H., Dhavale, D.D., Chopade, B.A. & Sachdev, D.P. 2014. Characterization and Fungal Inhibition Activity of Siderophore from Wheat Rhizosphere associated Acinetobacter calcoaceticus strain HIRFA32. Indian Journal of Microbiology, 54(3), 315-322.
  14. Mhatre, P.H., Karthik, C., Kadirvelu, K., Divya, K.L., Venkatasalam, E.P., Srinivasan, S., Ramkumar, G., Saranya, C. & Shanmuganathan, R. 2018. Plant Growth Promoting Rhizobacteria (PGPR): a Potential Alternative Tool for Nematodes Bio-Control. Biocatalysis and Agricultural Biotechnology, 17(3), 119–128.
  15. Raaijmakers, J. M. & Mazzola, M. 2012. Diversity and Natural Functions of Antibiotics produced by Beneficial and Plant Pathogenic Bacteria. Annual Review of Phytopathology, 50, 403–424.
  16. Sang M.K., Kim H.S., Myung I.S., Ryu C.M., Kim B.S. & Kim K.D. 2013. Chryseobacterium kwangjuense sp. nov. Isolated from Pepper (Capsicum annuum L.) root. International Journal of Systematic and Evolutionary Microbiology, 63, 2835–2840.
  17. Sang, M.K., Jeong, J.J. & Kim, K.D. 2018. Growth Promotion and Root Colonisation in Pepper Plants by Phosphate- Solubilising Chryseobacterium sp. strain ISE14 that suppresses Phytophthora blight. Annals of Applied Biology, 172(2), 208-223.
  18. San-Lang, W., Shih, I.L., Wang, C.H., Tseng, K.C., Chang, W.T., Twu, Y.K., Ro, J.J. & Wang, C.L. 2002. Production of Antifungal Compounds from Chitin by Bacillus subtilis. Enzyme and Microbial Technolnology, 31(3), 321-328.
  19. Saritha, B., Panneerselvam, P. & Ganeshamurthy, A.N. 2015. Antagonistic Potential of Mycorrhiza associated Pseudomonas putida against Soil Borne Fungal Pathogens. Plant Archives, 15(2), 763-768.
  20. Syed-Ab-Rahman, S. F., Carvalhais, L.C., Chua, E.T., Chung, F.Y., Moyle, P.M., Eltanahy, E.G. & Schenk, P.M. 2019. Soil Bacterial Diffusible and Volatile Organic Compounds inhibit Phytophthora capsici and promote Plant Growth. Science of The Total Environment, 692, 267-280.
  21. Wee, S.S., Musa,, I. B., Yong, K., Ling, K.W.S. & Nissom, P. M. 2021. Evaluation of Mycolytic Enzymes Producing Bacteria and Their Potentials as Biocontrol Agents against Ganoderma boninense. Borneo Journal of Science and Technology, 3(1), 51-60.
  22. Yang, M.M., Xu, L.P., Xue, Q.Y., Yang, J.H., Xu, Q., Liu, H.X. & Guo, J.H. 2012. Screening Potential Bacterial Biocontrol Agents towards Phytophthora capsici in Pepper. European Journal of Plant Pathology, 134(4), 811-820.
  23. Zhao, L.F., Xu, Y.J. & Lai, X.H. 2018. Antagonistic Endophytic Bacteria associated with Nodules of Soybean (Glycine max L.) and Plant Growth- Promoting Properties. Brazilian Journal of Microbiology, 49(2). 269-278.