In vitro Seed Germination of Coelogyne asperata Lindl. (Orchidaceae)

Arnnyitte Alexander; Jualang Azlan Gansau; Devina David.

Transactions on Science and Technology, 8(1), 1 - 6.

Back to main issue

ABSTRACT
A protocol for in vitro seed germination of Coelogyne asperata Lindl. has been established successfully. Immature seeds from 182 days old capsule were cultured on three different basal media; Murashige and Skoog (MS), Knudson C (KC), and Vacin and Went (VW) and maintained under continuous light at 25 ± 2 ˚C. After 30 days of culture (DAC), more than 90% of seeds were germinated on KC and VW media and about 84% of seeds germinated on MS medium. The incorporation of organic additives, including coconut water, potato homogenate and tomato juice each at 10% (v/v) in KC medium was tested to determine their effect on seed germination of C. asperata. The result revealed that KC basal medium alone without addition of organic additives promoted over 90% of seed germination at 30 DAC. Therefore, the protocol of using standard KC basal medium for C. asperata seeds germination could be suggested for mass propagation and conservation of this wild scented orchid.

KEYWORDS: Orchidaceae, Asymbiotic seed germination, Knudson C, Organic additives.



Download this PDF file

REFERENCES
  1. Abraham, S., Augustine, J. & Thomas, T. D. 2012. Asymbiotic seed germination and in vitro conservation of Coelogyne nervosa A. Rich. an endemic orchid to Western Ghats. Physiology and Molecular Biology of Plants, 18(3), 245–251. https://doi.org/10.1007/s12298-012-0118-6.
  2. Arditti, J., Clements, M. A, Fast, G., Hadlley, G., Hishimura, G. & Ernst, R. 1982. Orchid seed germination and seedling culture - A manual. In: Arditti, J. (ed.). Orchid biology: Review and prespective, Vol III. Cornell University, Press, Ithaca, New York.
  3. Bakar, B., Abdul L, M. & Gansau, J. A. 2014. Asymbiotic germination and seedling development of Dimorphics lowii (Orchidaceae). Asian Journal of Plant Biology, 2(1), 34–40.
  4. , R.A. 2001. Tracking rare orchids (Orchidaceae) in Arizona. Southwestern Rare and Endangered Plants: Proceedings of the Third Conference. 25 – 28 September 2000. Flagstaff, Arizona, pp.95-98.
  5. David, D., Jawan, R., Marbawi, H. & Gansau, J. A. 2015. Organic additives improves the in vitro growth of native orchid Vanda helvola Blume. Notulae Scientia Biologicae, 7(2), 192–197. https://doi.org/10.15835/nsb.7.2.9546.
  6. Daud, N., Taha, R.M., Noor, N.N.M. & Alimon, H. 2011. Provision of low cost media options for in vitro culture of Celosia sp. African Journal of Biotechnology, 10(80), 18349-18355.
  7. Araújo, S.D.S, Duque, A.S.R.L.A., Dos Santos, D.M.M.F. & Fevereiro, M.P.S. 2004. An efficient transformation method to regenerate a high number of transgenic plants using a new embryogenic line of Medicago truncatula cv. Jemalong. Plant Cell, Tissue and Organ Culture, 78(2), 123-131.
  8. Gansau, J. A., Indan, H., Abdullah, S. N., David, D., Marbawi, H. & Jawan, R. 2016. Effects of organic additives and plant growth regulators on protocorm development of Dendrobium lowii. Transactions on Science and Technology, 3(3), 462-468.
  9. Gravendeel, B. 2000. Reorganising the orchid genus Coelogene: A phylogenetic classification based on morphology and molecules. Doctoral Thesis, Leiden University, Netherland.
  10. Hartati, S., Arniputri, R. B., Soliah, L. A. & Cahyono, O. 2017. Effects of organic additives and naphthalene acetid acid (NAA) application on the in vitro growth of Black orchid hybrid (Coelogyne pandurata Lindley). Journal of Agricultural Science, 23(6), 951-957.
  11. Hinsley, A., De Boer, H. J., Fay, M. F., Gale, S. W., Gardiner, L. M., Gunasekara, R. S., Kumar, P., Masters, S., Metusala, D., Roberts, D. L., Veldman, S., Wong, S. & Phelps, J. 2018. A review of the trade in orchids and its implications for conservation. Botanical Journal of the Linnean Society, 186(4), 435–455. https://doi.org/10.1093/botlinnean/box083.
  12. Huh, Y. S., Lee, J. K., Nam, S. Y., Paek, K. Y. & Suh, G. U. 2016. Improvement of asymbiotic seed germination and seedling development of Cypripedium macranthos Sw. with organic additives. Journal of Plant Biotechnology, 43(1), 138–145. https://doi.org/10.5010/JPB.2016.43.1.138.
  13. Jualang, A. G., Devina, D., Hartinie, M., Sharon, J. S. & Roslina, J. 2014. Asymbiotic seed germination and seedling development of Vanda dearei. Malaysian Applied Biology, 43(2), 25-33.
  14. Kaur, S. & Bhutani, K. K. 2014. In vitro conservation and asymbiotic propagation of Coelogyne flaccida (Lindl.): A threatened orchid. Plant Biosystems - An International Journal Dealing with All Aspects of Plant Biology, 148(5), 935–944. https://doi.org/10.1080/11263504.2013.801368.
  15. Knudson, L. 1946. A new nutrient solution for the germination of orchid seed. American Orchid Society Bulletin, 15, 214-217.
  16. Koirala, D., Pradhan, S. & Pant, B. 2013. Asymbiotic seed germination and plantlet development of Coelogyne fuscescens Lindl., a medicinal orchid of Nepal. Scientific World, 11(11), 97-100.
  17. Lestari, N. K. D. 2015. Determining accurate harvesting times of Coelogyne asperata Lindl. seed capsules for propagation using tissue culture technique. Acta Horticulturae, 1078, 49-52.
  18. Lo, S.F., Nalawade, S.M., Kuo, C.L., Chen, C.L. & Tsay, H.S. 2004. Asymbiotic germination of immature seeds, plantlet development and ex vitro establishment of plants of Dendrobium tosaense makino-A medicinally important orchid. In Vitro Cellular & Developmental Biology-Plant, 40(5), 528-535.
  19. Murashige, T. & Skoog, F. 1962. A revised medium for rapid growth and bioassay with tobacco tissue culture. Physiologia Plantarum, 15(3), 473-497.
  20. Rasmussen, H. N., Dixon, K. W., Jersáková, J. & Těšitelová, T. 2015. Germination and seedling establishment in orchids: A complex of requirements. Annals of Botany, 116(3), 391–402.
  21. Seaton, P.T., Hu, H., Perner, H. & Pritchard, H.W. 2010. Ex situ conservation of orchids in a warming world. The Botanical Review, 76(2), 193-203.
  22. Semiarti, E., Indrianto, A., Suyono, E. A., Nurwulan, R. L., Restiani, R., Machida, Y. & Machida, C. 2010. Genetic Transformation of the Indonesian Black Orchid (Coelogyne pandurata Lindley) through Agrobacterium tumefaciens for Micropropagation. Proceedings of Nagoya International Orchid Congress (NIOC), 2010. Nagoya Dome, Japan. pp 16-20.
  23. Suriya, K., Philip Robinson, J., Sebastinraj, J. & Kakati, J. P. 2017. In vitro seed germination of Cymbidium aloifolium (L.) Sw., a potential medicinal Orchid from Eastern Ghats of Tamil Nadu, India. Journal of Plant Biotechnology, 44(3), 343–348. https://doi.org/10.5010/JPB.2017.44.3.343.
  24. Srivastava, D., Gayatri, M.C. & Sarangi, S.K. 2015. In vitro seed germination and plant regeneration of an epiphytic orchid Aerides ringens (Lindl.) Fischer. Indian Journal of Biotechnology, 14, 574-580.
  25. Vacin, E. F. & Went, F. W. 1949. Some pH changes in nu¬trient solutions. Botanical Gazette, 110, 604-613.
  26. Wood, J. J. & Cribb, P. J. 1995. A Checklist of the Orchids of Borneo. Royal Botanic Garden, Kew.
  27. Yam T.W., Nair H., Hew C.S., Arditti J. 2002. History-Seeds. In: Kull T., Arditti J. (eds) Orchid Biology: Reviews and Perspectives, VIII. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-2500-2_7.