Cytotoxicity and Acute Oral Toxicity of Ascomycetous Mycoparasitic Scytalidium parasiticum
Yit Kheng Goh, Nurul Fadhilah Marzuki, Choon Kiat Lim, You Keng Goh, Kah Joo Goh
ABSTRACT: Scytalidium parasiticum (anamorph of Xylogone species) was described and reported as potential necrotrophic mycoparasite of Ganoderma boninense in two previous studies. This mycoparasitic S. parasiticum showed capability in reducing Ganoderma disease incidences and severity in the nursery. However, the scientific information on toxicity of S. parasiticum is limited. In the current study, S. parasiticum has been selected for cytotoxicity (MTT assay – yellow tetrazolium salt) and acute oral toxicity in rodent tests to determine the toxicity level of S. parasiticum. There were 6 different concentrations of lyophilized S. parasiticum tested – 0.063, 0.125, 0.25, 0.5, 1, and 2 mg/mL, in MTT assay. Results from MTT assay illustrated that S. parasiticum was considered to be weakly cytotoxicity (Inhibition concentration of 50% at the dose of 2 mg/mL) in V79-4 cells (Chinese hamster lung cells). There was no adverse toxic reaction observed in acute oral toxicity test as well (using Female Sprague-Dawley rats) at the rate of 2000 mg/kg body weight. The findings of the current study demonstrated that Scytalidium parasiticum was observed to be relatively safe with low to very low cell cytotoxicity effects and appeared to be none toxic to rats.
KEYWORDS: Basal stem rot; Ganoderma boninense; inhibition concentration; necrotrophic mycoparasite
Received
3 November 2016
Revised
16 November 2016
Accepted
18 November 2016
Online
20 December 2016
© Transactions on Science and Technology 2016
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