Extraction and Characterization of Chitosan from Shrimp Shell Waste in Sabah

Flornica Alca Ahing; Newati Wid

Extraction and Characterization of Chitosan from Shrimp Shell Waste in Sabah

Flornica Alca Ahing & Newati Wid

Keywords: Extraction; Chitosan; Moisture Content; Solubility; Degree of Deacetylation

A b s t r a c t

Chitin is the most widespread renewable natural sources following cellulose and the main source of chitin is crustacean waste. Chitosan which is a derivative of chitin after the process of deacetylation has multiple of commercial and possible medical uses based on its degree of deacetylation. This research aims to study the production of chitosan from shrimp shell waste in Sabah and characterize the chitosan quality which includes parameters including moisture content, solubility, and degree of deacetylation (DDA). The results obtained from this study show that moisture content ranged from 4-7%, while the solubility of chitosan achieved up to 90%. The DDA value obtained was high ranged from 70-85%. Based on these three characteristics, shrimp shell waste in Sabah can achieved chitosan standard quality for industrial application by performing traditional method of deproteination, demineralization and deacetylation.

© Transactions on Science and Technology 2016

References

[1] Abdou, E. S., Nagy, K. S. A. & Elsabee, M. Z. (2008). Extraction and Characterization of Chitin and Chitosan from Local Sources. Bioresource Technology, 99, 1359-1368.

[2] Abdulkarim, A., Isa, M. T., Abdulsalam, S., Muhammad, A. J. & Ameh, A. O. (2013). Extraction and Characterization of Chitin and Chitosan from Mussel Shell. Civil and Environmental Research, 3(2), 108-114.

[3] Al-Sagheer, F. A., Al-Sughayer, M. A., Muslim, S. & Elsabee, M. Z. (2009). Extraction and Characterization of Chitin and Chitosan from Marine Sources in Arabian Gulf. Carbohydrate Polymers, 77, 410-419.

[4] Alishahi, A., Mirvaghefi, A., Tehrani, M. R., Farahmunh, H., Shojaosadati, S. A., Dorkoosh, F. A. & Maher, Z. E. (2011). Enhancement and Characterization of Chitosan Extraction from the Wastes of Shrimp Packaging Plants. Journal Polymer Environment, 19, 776-783

[5] Blagodatskikh, I. V., Kulikov, S. N., Vyshivannaya, O. V., Bezrodnykh, E. A., Yamskov, I. A. & Tikhonov, V. E. (2013). Influence of Glucosamine on Oligochitosan Solubility and Antibacterial Activity. Carbohydrate Research, 381, 28-32.

[6] Brugnerotto, J., Lizardi, J., Goycoolea, F. M., Arguelles-Monal, W., Desbrieres, J. & Rinaudo, M. (2001). An Infrared Invertigation in Relation with Chitin and Chitosan Characterization. Polymer, 42, 3569-3580.

[7] Coates, J. (2000). Interpretation of Infrared Spectra, A Practical Approach. In: Meyers, R.A. (ed). Encyclopedia of Analytical Chemistry. John Wiley & Sons.

[8] Esam, A. E., Abdul, H. Y. & Misni, M. (2009). Characterisation of Chitosan Solubilised in Aqueous Formic and Acetic Acid. Journal of Science and Technology, 3(03), 415-425.

[9] Prashanth, K. V. H. & Tharanathan, R. N. (2007). Chitin/chitosan: Modifications and Their Unlimited Application Potential- An Overview. Trends in Food Science and Technology, 18, 117-131.

[10] Hussain, M. R., Iman, M. & Maji, T. K. (2013). Determination of Degree of Deacetylation of Chitosan And their Effect on the Release Behaviour Of essential Oil from Chitosan and Chitosan Gelatin Complex Microcapsules. International Journal of Advanced Engineering Applications, 6(4), 4-12.

[11] Jitareerat, P., Paumchai, S., Kanlayanarat, S. & Sangchote, S. (2007). Effect Of Chitosan On Ripening, Enzymatic Activity, And Disease Development In Mango (Mangifera Indica) Fruit. New Zealand Journal of Crop and Horticultural Science, 35 (2), 211-218.

[12] Kumari, S. & Rath, P. K. (2014). Extraction and Characterization of Chitin and Chitosan from (Labeo Rohit) Fish Scales. Procedia Material Science, 6, 482-489.

[13] Naznin, R. (2005). Extraction of Chitin and Chitosan from Shrimp (Metapenaeus monoceros) Shell by Chemical Method. Pakistan Journal of Biological Science, 8(7), 1051-1054.

[14] Nouri, M. Khodaiyan, F., Razavi, S. H. & Mousavi, M. (2015). Improvement of Chitosan Production from Persian Gulf Shrimp Waste by Response Surface Technology. Food Hydrocolloids, 3, 1-9.

[15] Pal, J., Verma, H. O., Munka, V. K., Maurya, S. K., Roy, D. & Kumar, J. (2014). Biological Method of Chitin Extraction from Shrimp Waste An eco-Friendly Low Cost Technology and Its Advanced Application. International Journal of Fisheries and Aquatic Studies, 1(6), 104-107.

[16] Patria, A. (2013). Production and Characterization of Chitosan from Shrimp Shells Waste. Bioflux, 6,4.

[17] Pillai, C. K. S., Paul, W. & Sharma, C. P. (2009). Chitin and Chitosan Polymers: Chemistry, Solubility and Fiber Formation. Progress in Polymer Science, 34, 641-678.

[18] Puvvada, Y. S., Vankayalapati, S. & Sukhavasi, S. (2012). Extraction of Chitin from Chitosan Exoskeleton of Shrimp for Application in the Pharmaceutical Industry. International Current Pharmaceutical Journal, 1(9), 258-263.

[19] Rinaudo, M. (2006). Chitin and Chitosan: Properties and Applications. Progress Polymer Science, 31, 603-632.

[20] Szymanska, E. & Winnicka, K. (2015). Stability of Chitosan – A Challenge for Pharmaceutical and Biomedical Application. Marine Drugs, 13, 1819-1864.

[21] Viljoen, J. M., Steekamp, J. H., Marais, A. F. & Kotze, A. F., (2014). Effect of moisture content, temperature and exposure time on the physical stability of chitosan powder and tablets. Drug Development Industrial Pharmaceuticals, 40, 730-742.