GC-MS Analysis of Strobilanthes crispus Plants and Callus |
Bo Eng Cheong, Nur Aina Zakaria, Angelina Ying Fang Cheng & Peik Lin Teoh |
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Keywords: GC-MS; Strobilanthes crispus; callus |
A b s t r a c t |
Strobilanthes crispus or locally known as “bayam karang”, “pecah kaca”, “jin batu” and “pecah beling” in Malaysia, has been traditionally used to increase immune system, treating kidney stones, treatment of diabetes mellitus, treatment of high blood pressure and treatment of wound. Studies examining the phytochemical constituents reported that the leaves of this plant contain ester glycosidic compound of caffeic acid, -voumaric acid, , vanilic acid, ferulic acid, syringic acids, sitosterol, campesterol, hexadecanoic acid, methylester, lupeol, phytol, stigmasterol, flavonoid compounds such as (+)-catechin, (-)-epicatechin, rutin, and etc. While most of the literatures focused on the chemical compounds present in the leaves of
S. crispus, none have been reported for the phytochemical constituents of the whole
S. crispus plant including the leaf, stem, root or flower part. Besides, there is also lacking report on the tissue culture generated from this plant too. Thus, this study was carried out to profile the leaves, stems and roots and callus cultures of
S. crispus using gas chromatography mass spectrometry (GC-MS) approach. Results revealed that this plant is rich with squalene, phytosterols such as stigmasterols and derivatives, sito-sterol, campesterols, as well as triterpenoids such as lupeol, amyrin and betulin. © Transactions on Science and Technology 2016 |
References
[1]
Atif, B. A., Heinric, W. & Carol, S. F. (2003). Effect of
phytosterols on cholesterol metabolism and MAP kinase in
MDA-MB-231 human breast cancer cells.
The
Journal of Nutritinal
Biochemistry, 14(2),
111-119.
[2]
Baker, C. A. D. & Bakhuizen, R. C. V. (1965). Flora of Java
(spermatophytes only). Volume II. Griningen: Noordhoff Press.
[3]
Ghosh, T., Maity, T. K. & Singh, J. (2011). Evaluation of
antitumor activity of stigmasterol, a constituent isolated from
Bacopa monnieri Linn
aerial parts against Ehrlich Ascites Carcinoma in mice.
Oriental Pharmacy and
Experimental Medicine,
11(1), 41–49.
[4]
Liza, M. S., Abdul Rahman, R., Mandana, B., Jinap, S., Rahmat,
A., Zaidul, I. S. M & Hamid, A. (2010). Supercritical carbon
dioxide extraction of bioactive flavonoid from
Strobilanthes crispus (Pecah kaca).
Food and Bioproducts Processing,
88(2-3), 319-326.
[5]
Milne. A. & Beamish T. (1999). Inhalation and local anesthetics reduce tactile and thermal responses in
Mimosa pudica. Canadian
Journal of Anesthesia, 46(3), 287-289.
[6]
Muslim, N. S., Ng, K. W., Itam, A., Nassar, Z. D., Ismail, Z.
& Abdul Majid, A. M .S. (2010). Evaluation of cytotoxic,
anti-angiogenic and antioxidant properties of standardized
extracts of Strobilanthes crispus leaves.
International Journal of Pharmacology,
6(5), 591-599.
[7]
Ong, H. C. and Norzalina, J. 1999. Malay herbal medicine in
Gemencheh, Negeri Sembilan, Malaysia.
Fitoterapia,
70(1), 10 - 14.
[8]
Passosa, G. F., Fernandesa, E. S., da Cunha, F. M., Ferreira, J., Pianowski, L. F. & Calixto, J. B.
(2007). Anti-inflammatory and anti-allergic properties of the
essential oil and active compounds from
Cordia verbenacea.
Journal of Ethnopharmacology,
110(2), 323–333.
[9]
Smith, T. J. (2000). Squalene: potential chemopreventive agent.
Expert Opinion
on Investigational Drugs,
9(8),
1841–1848.
[10]
Yaacob, N. S., Hamzah, N., Kamal, N. M., Zainal Abidin, S. A.,
Lai, C. S., Navaratnam, V. & Norazmi, M. N. (2010). Anti-cancer
activity of sub-fraction of dichloromethane extract of
Strobilathes crispus on
human breast and prostate cancer cells in vitro.
BMC Complement Alternative
Medicine, 10, 1-14. |