Rapid Detection of Allura Red (E129) Based on Chitosan/Nanoparticles/MWCNTs Modified Gold Electrode in Food Products

Rovina KOBUN, Shafiquzzaman SIDDIQUEE & Sharifudin Md SHAARANI

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Keywords: Allura red; Chitosan; Multiwall carbon nanotubes; Electrochemical.

A b s t r a c t
Allura Red AC (E 129) is a mono azo dye that is able to form of powder or granules. It is used to make foods more attractive and appetizing for over centuries. However, excessively intake of food containing synthetic food coloring can caused toxicity and pathogenicity to human. Therefore, there is needed a simple, faster and effective method for the analysis of Allura Red in food products. Herein, an electrochemical sensor for rapid and simple detection of Allura Red was developed based on combination of nanomaterials of chitosan, nanoparticles and multiwall carbon nanotubes (MWCNTs) sensing film. Methylene blue was used as a redox indicator for increasing the electron transfer in electrochemical cell. The electrochemical behavior of modified gold electrode in the presence of Allura Red was studied by using cyclic voltammetries and differential pulse voltammetries. The morphological characteristics of modified electrode were observed under scanning electron microscope and transmission electron microscope. Under optimal conditions, this electrochemical method was found a wide linear Allura Red concentrations range from 10 to 0.5 ppm, with a detection limit are as low as 0.4 ppm. Finally, the proposed sensor method was successfully used to detect Allura Red in different sample food products.

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