Effects of organic acid treatments on selected quality parameters of green mussels (Perna viridis) during cold storage

Wei Chen Oh; Jau Shya Lee; Julian Ransangan; Chee Kiong Siew.

Green mussels (Perna viridis), a bivalve, are found in Malaysia and other regions of Asia-Pacific. They are rich in protein, polyunsaturated fatty acids, minerals and vitamins. However, green mussels are subject to rapid post-harvest quality deterioration that leads to spoilage and safety concerns. Effective post-harvest treatments are therefore critical in the quality preservation of green mussels. In the present study, the effects of organic acids (lactic acid, oxalic acid, and tartaric acid), either single-acid or combination-acid at 1.0% concentration, on the quality of green mussels under cold storage (4 ± 1 °C) for 20 days were studied. Untreated mussels were used as the control. All organic acid-treated mussels showed lower total viable counts and psychrotrophic bacteria counts than the control throughout the cold storage. The application of organic acids reduced the production of volatile nitrogenous compounds resulting in lower total volatile base-nitrogen (TVB-N) and trimethylamine (TMA) contents in treated mussels. In conclusion, organic acid treatments exhibited effective antimicrobial activity and reduced the production of volatile nitrogenous compounds during cold storage, suggesting their potential to preserve the quality and extend the shelf life of green mussels.

KEYWORDS: Cold storage; Green mussel; Organic acid; Quality.



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REFERENCES

1. Amrutha, B., Sundar, K. & Shetty, P. H. 2017. Effect of organic acids on biofilm formation and quorum signaling of pathogens from fresh fruits and vegetables. Microbial Pathogenesis, 111, 156-162.
2. Anang, D. M., Rusul, G. & Moda, H. M. 2015. Inhibitory effects of oxalic acid on Listeria monocytogenes, Salmonella Enteritidis and Escherichia coli O157:H7 inoculated onto chicken breast stored at 4 °C.
International Journal of Food Science, Nutrition and Dietetics, 04(6), 233-238.
3. AOAC. 2000. Official Methods of Analysis of AOAC International. (17th edition). Washington DC: AOAC International.
4. Arcales, J. A. A. & Nacional, L. M. 2018. Effect of lactic and citric acid pretreatment on quality changes of green mussel (Perna viridis) during chilled storage. Current Research in Nutrition and Food Science, 6(3), 862-872.
5. Arcales, J. A. A. & Nacional, L. M. 2019. Quality changes of chilled green mussel (Perna viridis) pre-treated with organic acids and sodium tripolyphosphate. Current Research in Nutrition and Food Science, 7(3), 894-904.
6. Aru, V., Pisano, M. B., Savorani, F., Engelsen, S. B., Cosentino, S. & Marincola, F. C. 2016. Metabolomics analysis of shucked mussels’ freshness. Food Chemistry, 205, 58-65.
7. Baker, P., Fajans, J. S., Arnold, W. S., Ingri, D. A., Marelli, D. C. & Baker, S. M. 2007. Range and dispersal of a tropical marine invader, the Asian green mussel, Perna viridis, in subtropical waters of the Southeastern United States. Journal of Shellfish Research, 26(2), 345-355.
8. Boysen, L., Wechter, N. S. & Rosenquist, H. 2013. Effects of decontamination at varying contamination levels of Campylobacter jejuni on broiler meat. Poultry Science, 92(5), 1425-1429.
9. Bindu, J., Ginson, J., Kamalakanth, C. K. & Gopal, T. K. S. 2015. High pressure treatment of green mussel Perna viridis Linnaeus, 1758: Effect of shucking and quality changes in meat during chill storage. Indian Journal of Fish, 62(2), 70-76.
10. Chakraborty, K., Chakkalakal, S. J., Joseph, D., Asokan, P. K. & Vijayan, K. K. 2016. Nutritional and antioxidative attributes of green mussel (Perna viridis L.) from the Southwestern Coast of India. Journal of Aquatic Food Product Technology, 25(7), 968-985.
11. Erkan, N. 2005. Changes in quality characteristics during cold storage of shucked mussels (Mytilus galloprovincialis) and selected chemical decomposition indicators. Journal of the Science of Food and Agriculture, 85, 2625-2630.
12. Commission Regulation (EC) No 2074/2005. Annex II (Fishery Products). Section I (Obligations on Food Business Operations). Chapter III (Determination of the concentration of TVB-N in fish and fishery products). Official Journal of the European Union L338. pp 37-39.
13. Fernández, M., Rodríguez, A., Fulco, M., Soteras, T. Mozgovoj, M. & Cap, M. 2021. Effects of lactic, malic and fumaric acids on Salmonella spp. counts and on chicken meat quality and sensory characteristics. Journal of Food Science and Technology, 58, 3817-3824.
14. Goulas, A. E., Chouliara, I., Nessi, E., Kontominas, M. G. & Savvaidis, I. N. 2005. Microbiological, biochemical and sensory assessment of mussel (Mytilus galloprovincialis) stored under modified atmosphere packaging. Journal of Applied Microbiology, 98, 752-760.
15. Hassan, R., El-Kadi, S. & Sand, M. 2015. Effect of some organic acids on some fungal growth and their toxins production. International Journal of Advances in Biology (IJAB), 2(1), 1-11.
16. International Organization for Standardization (ISO). 2013. ISO 4833-1:2013. Microbiology of the Food Chain – Horizontal Method for the Enumeration of Microorganisms. Part 1: Colony Count at 30°C by the Pour Plate Technique. Switzerland: ISO.
17. International Organization for Standardization (ISO). 2019. ISO 17410:2019. Microbiology of the Food Chain – Horizontal Method for the Enumeration of Psychrotrophic Microorganisms. Switzerland: ISO.
18. Khalafalla, F. A., Ali, F. H. M. & Hassan, A. H. A. 2016. Quality improvement of broiler chicken breasts by nisin and lactic acid. Journal of World’s Poultry Research, 6(2), 37-47.
19. Leon Peláez, A. M., Serna Cataño, C. A., Quintero Yepes, E. A., Gamba Villarroel, R. R., De Antoni, G. L. & Giannuzzi, L. 2012. Inhibitory activity of lactic and acetic acid on Aspergillus flavus growth for food preservation. Food Control, 24, 177-183.
20. Manju, S., Jose, L., Gopal, T. K. S., Ravishankar, C. N. & Lalitha, K. V. 2007. Effects of sodium acetate dip treatment and vacuum packaging on chemical, microbiological, textural and sensory changes of pearlspot (Etroplus suratensis) during chill storage. Food Chemistry, 102, 27-35.
21. Masniyom, P., Benjama, O. & Maneesri, J. 2011. Extending the shelf-life of refrigerated green mussel (Perna viridis) under modified atmosphere packaging. Songklanakarin Journal of Science and Technology, 33(2), 171-179.
22. Mohamed, M. H. & Ammar, M. A. M. 2021. Effect of some antimicrobials on quality and shelf life of freshwater tilapia (Oreochromis niloticus). Journal of Food Processing and Preservation, 45(1), e15026.
23. Over, K. F., Hettiarachchy, N., Johnson, M. G. & Davis, B. 2009. Effect of organic acids and plant extracts on Escherichia coli O157:H7, Listeria monocytogenes, and Salmonella Typhimurium in broth culture model and chicken meat systems. Journal of Food Science, 74(9), M515-M521.
24. Prapaiwong, N., Wallace, R. K. & Arias, C. R. 2009. Bacterial loads and microbial composition in high pressure treated oysters during storage. International Journal of Food Microbiology, 131, 145-150.
25. Ramanathan, R., Mancini, R. A. & Dady, G. A. 2011. Effects of pyruvate, succinate, and lactate enhancement on beef longissimus raw color. Meat Science, 88, 424-428.
26. Sallam, K. I. 2007. Chemical, sensory and shelf life evaluation of sliced salmon treated with salts of organic acids. Food Chemistry, 101, 592-600.
27. Smyth, C., Brunton, N. P., Fogarty, C. & Bolton, D. J. 2018. The effect of organic acid, trisodium phosphate and essential oil component immersion treatments on the microbiology of cod (Gadus morhua) during chilled storage. Foods, 7(12), 200.
28. Tavares, J., Martins, A., Fidalgo, L. G., Lima, V., Amaral, R. A., Pinto, C. A., Silva, A. M. & Saraiva, J. A. 2021. Fresh fish degradation and advances in preservation using physical emerging technologies. Foods, 10, 780.
29. Velammal, A., Saritha, K., Immaculate, J. K. & Jamila, P. 2017. Food safety and shelf life of cooked meat of brown mussel, Perna perna (Linnaeus, 1758), stored under different temperatures. International Food Research Journal, 24(6), 2609-2615.