Synergistic effects of Tiger Milk Mushroom and Long Jack on endurance running performance and selected physiological responses in athletes

Chee Ping Fadzel Wong; Lau Ngikk Ling.

The consumption of ergogenic supplements is a common strategy among athletes to enhance sports performance. Tiger Milk Mushroom and Long Jack are traditional herbal supplements known for their potential health and physical fitness benefits, and their use has recently gained attention in the sports industry. However, there is currently limited scientific evidence regarding the effects of these supplements on endurance running performance. This study aimed to investigate the effects of combined Tiger Milk Mushroom and Long Jack supplementation on endurance running performance and selected physiological parameters in athletes. Eight male athletes participated in this randomized cross-over study. Participants consumed either a combination of Tiger Milk Mushroom and Long Jack or a placebo for six consecutive days prior to the endurance running test. On the day of the experimental trial, the supplement or placebo was administered one hour before the test. The time to complete the running test, heart rate, rate of perceived exertion (RPE), maximal oxygen consumption (VO₂max), blood pressure, and body weight were measured in both trials. A one-week washout period was implemented between trials. Data were expressed as mean ± standard deviation. Repeated-measures ANOVA and paired t-tests were used for statistical analysis. This study found no significant differences in the time to complete the running test, VO₂max, heart rate, RPE, blood pressure and body weight between the two trials (p >0.05). Heart rate, RPE and blood pressure significantly increased from resting values in both trials (p < 0.05). In conclusion, six days of combined Tiger Milk Mushroom and Long Jack supplementation, including an additional dose one hour before the endurance running test, did not produce significant ergogenic effects on endurance running performance and selected physiological parameters in athletes.

KEYWORDS: Tiger Milk Mushroom; Long Jack, Endurance running; Heart rate; Maximum oxygen consumption.



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REFERENCES

1. American College of Sports Medicine (ACSM). 2021. ACSM’s Guidelines for Exercise Testing and Prescription (11th ed.). Wolters Kluwer.
2. Bhat, R. & Karim, A. A. 2010. Tongkat Ali (Eurycoma longifolia Jack): A review on its ethnobotany and pharmacological importance. Fitoterapia, 81(7), 669–679.
3. Braakhuis, A. J. & Hopkins, W. G. 2015. Impact of dietary antioxidants on sport performance: A review. Sports Medicine, 45(7), 939–955.
4. Brauer, P., Anielski, P., Schwaiger, S., Stuppner, H., Tran, T. V. A., Vollmer, G., Zierau, O., Thieme, D. & Keiler, A. M. 2019. In vitro metabolism of selected bioactive compounds of Eurycoma longifolia root extract to identify suitable markers in doping control. Drug Testing and Analysis, 11(1), 86–94.
5. Chen, C. K., Hamdan, N. F., Ooi, F. K. & Wan Abd Hamid, W. Z. 2016. Combined Effects of Lignosus rhinocerotis supplementation and resistance training on isokinetic muscular strength and power, anaerobic and aerobic fitness level, and immune parameters in young Males. International Journal of Preventive Medicine, 7, 107.
6. Chen, C. K., Mohamad, W. M., Ooi, F. K., Ismail, S. B., Abdullah, M. R. & George, A. 2014. Supplementation of Eurycoma longifolia Jack extract for 6 weeks does not affect urinary testosterone: epitestosterone ratio, liver and renal functions in male recreational athletes. International Journal of Preventive Medicine, 5(6), 728–733.
7. Chen, C. K., Muhamad, A. S. & Ooi, F. K. 2012. Herbs in exercise and sports. Journal of Physiological Anthropology, 31(1), 4.
8. Fung, S. Y. & Tan, C. S. 2017. The Bioactivity of Tiger Milk Mushroom: Malaysia’s Prized Medicinal Mushroom. In: Agrawal, D.C., Tsay, H-S., Shyur, L-F., Wang, S-Y. (Eds.). Medicinal Plants and Fungi: Recent Advances in Research and Development. Singapore: Springer.
9. Hamzah, S. & Yusof, A. 2003.The ergogenic effects of Eurycoma longifolia Jack: A pilot study. British Journal of Sports Medicine, 37, 464-470.
10. Harty, P. S., Zabriskie, H. A., Erickson, J. L., Molling, P. E., Kerksick, C. M. & Jagim, A. R. 2018. Multi-ingredient pre-workout supplements, safety implications, and performance outcomes: a brief review. Journal of the International Society of Sports Nutrition. 15(1), 41.
11. Henkel, R. R., Wang, R., Bassett, S. H., Chen, T., Liu, N., Zhu, Y. & Tambi, M. I. 2014. Tongkat Ali as a potential herbal supplement for physically active male and female seniors—a pilot study. Phytotherapy Research, 28(4), 544-550.
12. Higgins, M. R., Izadi, A. & Kaviani, M. 2020. Antioxidants and exercise performance: with a focus on Vitamin E and C supplementation. International Journal of Environmental Research and Public Health, 17(22), 8452.
13. Khanijo, T. & Jiraungkoorskul, W. 2016. Review ergogenic effect of Long Jack, Eurycoma Longifolia. Pharmacognosy Reviews, 10(20), 139–142.
14. Kraemer, W. J. & Ratamess, N. A. 2005. Hormonal responses and adaptations to resistance exercise and training. Sports Medicine, 35, 339-361.
15. Lau, B. F., Abdullah, N. & Aminudin, N. 2013. Chemical composition of the tiger’s milk mushroom, Lignosus rhinocerotis (cooke) Ryvarden, from different developmental stages. Journal of Agricultural and Food Chemistry, 61(20), 4890-4897.
16. Muhamad, A. S., Keong, C. C., Kiew, O. F., Abdullah, M. R., & Lam, C. T. 2010. Effects of Eurycoma longifolia Jack supplementation on recreational athletes' endurance running capacity and physiological responses in the heat. International Journal of Apply Sports Science, 22(2), 1-19.
17. Nallathamby, N., Phan, C. W., Seow, S. L., Baskaran, A., Lakshmanan, H., Abd Malek, S. N. & Sabaratnam, V. 2018. A status review of the bioactive activities of tiger milk mushroom Lignosus rhinocerotis (Cooke) Ryvarden. Frontiers in Pharmacology, 8, 998.
18. Ooi, F. K., Singh, R., Sirisinghe, R. G., Ang, B. & Jamalullail, S. 2001. Effects of an herbal ergogenic drink on cycling performance in young cyclists. Malaysian Journal of Nutrition, 7(1), 33–40.
19. Rehman, S. U., Choe, K. & Yoo, H. H. 2016. Review on a traditional herbal medicine, Eurycoma longifolia Jack (Tongkat Ali): its traditional uses, chemistry, evidence-based pharmacology and toxicology. Molecules, 21(3), 331.
20. Simioni, C., Zauli, G., Martelli, A. M., Vitale, M., Sacchetti, G., Gonelli, A. & Neri, L. M. 2018. Oxidative stress: Role of physical exercise and antioxidant nutraceuticals in adulthood and aging. Oncotarget, 9(24), 17181–17198.
21. Talbott, S. M., Talbott, J. A., George, A. & Pugh, M. 2013. Effect of Tongkat Ali on stress hormones and psychological mood state in moderately stressed subjects. Journal of the International Society of Sports Nutrition, 10(1), 28.
22. Talbott, S., Talbott, J., Negrete, J., Jones, M., Nichols, M. & Roza, J. 2006. Effect of Eurycoma longifolia extract on anabolic balance during endurance exercise. Journal of the International Society of Sports Nutrition, 3(1), S32.
23. Tambi, M. I. B. M., Imran, M. K. & Henkel, R. R. 2012. Standardised water‐soluble extract of Eurycoma longifolia, Tongkat Ali, as testosterone booster for managing men with late‐onset hypogonadism. Andrologia, 44, 226-230.
24. Tan, E. S. S., Leo, T. K. & Tan, C. K. 2021. Effect of tiger milk mushroom (Lignosus rhinocerus) supplementation on respiratory health, immunity and antioxidant status: an open-label prospective study. Scientific Reports, 11(1), 11781.
25. Wong, X.K., Alasalvar, C., Ng, W.J., Ee, K.Y., Lam, M.Q. & Chang S. K. 2024. Tiger milk mushroom: A comprehensive review of nutritional composition, phytochemicals, health benefits, and scientific advancements with emphasis on chemometrics and multi-omics. Food Chemistry, 459, 140340.
26. Yap, H. Y., Aziz, A. A., Fung, S. Y., Ng, S. T., Tan, C. S. & Tan, N. H. 2014. Energy and nutritional composition of Tiger milk mushroom sclerotia and the antioxidant activity of its extracts. International Journal of Medical Sciences, 11(6), 602–607.
27. Zakaria, A.Z., Washif, J.A., Lim, B.H. & Nosaka, K. 2023. Effects of Eurycoma longifolia Jack supplementation on eccentric leg press exercise-induced muscle damage in rugby players. Biology of Sport, 40(3), 691-697.