DDT-Pyrethroid Resistance Screening in Association with the kdr Allele F1534C in Aedes albopictus (Skuse) (Diptera: Culicidae)

Azlinda Abu Bakar; Amani Ahmad Mokhtar; Tuan Nur Akmalina Mat Jusoh; Rafidah Hanim Shueb.

The emergence of insecticide resistance in Aedes against pyrethroid group has become a threat to the vector control program. This study investigates the resistance status and the presence of F1534C kdr mutations in Aedes albopictus populations of Kota Bharu and Kubang Kerian, Kelantan, Malaysia. The F1 adults of Ae. albopictus were assayed using World Health Organization (WHO) susceptibility test with 4% dichloro-diphenyl-trichloroethane (DDT), 0.05% lambda-cyhalothrin, and 0.75% permethrin. For susceptibility analysis, the mortality percentage, 50% cumulative knockdown time (KT50), and resistance ratio (RR) values were calculated. All the mosquito survivors were collected and subjected to the allele-specific polymerase chain reaction (AS-PCR) analysis on the presence of knockdown resistance (kdr) mutation F1534C. Results show that Ae. albopictus from Kota Bharu was possible resistance to DDT and pyrethroids, while Ae. albopictus in Kubang Kerian showed mixed resistant populations which are possible resistance and susceptibility to DDT and pyrethroids, respectively. The kdr alleles F/C1534 were detected in both Ae. albopictus populations with higher heterozygote resistant alleles (F/C) in Kota Bharu.

KEYWORDS: Ae. albopictus, kdr, F1534C, DDT, pyrethroids



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REFERENCES

1. Abbott, W.S. 1925. A method of computing the effectiveness of an insecticide. Journal of Economic Entomology, 18(2), 265-266.
2. Abu Bakar, A., Ahmad Mokhtar, A., Mat Jusoh, T.N.A. & Shomiad, R.H. 2021. Evaluation of the DDT and Pyrethroid Resistance Status of Aedes aegypti (Linnaeus) (Diptera: Culicidae) in Kota Bharu, Kelantan. Transactions on Science Technology, 8(3), 128-136.
3. Abu Bakar, A., Mohd Roslin, N.E.A., Mat Jusoh, T.N.A. & Shueb, R.H. 2018. A short survey on the distribution and abundance of Aedes spp in Universiti Sains Malaysia Kelantan in relation to dengue virus. Health and The Environment Journal, 9(1), 35-50.
4. Baldacchino, F., Caputo, B., Chandre, F., Drago, A., della Torre, A., Montarsi, F. & Rizzoli, A. 2015. Control methods against invasive Aedes mosquitoes in Europe: A review. Pest anagement Science, 71(11), 1471-1485.
5. Bisset, J.A., Marín, R., Rodríguez, M.M., Severson, D.W., Ricardo, Y., French, L., Díaz, M. & Pérez, O. 2013. Insecticide resistance in two Aedes aegypti (Diptera: Culicidae) strains from Costa Rica. Journal of Medical Entomology, 50(2), 352-361.
6. Finney, J. 1972. Probit analysis (3rd edition). London: Cambridge University Press.
7. Gratz, N.G. 2004. Critical review of the vector status of Aedes albopictus. Medical and Veterinary Entomology, 18(3), 215-227.
8. Ishak, I.H., Jaal, Z., Ranson, H. & Wondji, C.S. 2015. Contrasting patterns of insecticide esistance and nockdown resistance (kdr) in the dengue vectors Aedes aegypti and Aedes albopictus from Malaysia. Parasites Vectors, 8, 181.
9. Kraemer, M.U.G., Reiner, R.C., Brady, O.J., Messina, J.P., Gilbert, M., Pigott, D. M., Yi, D., Johnson, K., Earl, L., Marczak, L. B., Shirude, S., Weaver, N. D., Bisanzio, D., Perkins, T. A., Lai, S., Lu, X., Jones, P., Coelho, G. E., Carvalho, R. G., Van Bortel, W., Marsboom, C., Hendrickx, G., Schaffner, F., Moore, C. G., Nax, H. H., Bengtsson, L., Wetter, E., Tatem, A. J., Brownstein, J. S., Smith, D. L., Lambrechts, L., Cauchemez, S., Linard, C., Faria, N. R., Pybus, O. G., Scott, T. W., Liu, Q., Yu, H., William Wint, G. R., Hay, S. I. & Golding, N. 2019. Past and future spread of the arbovirus vectors Aedes aegypti and Aedes albopictus. Nature Microbiology, 4, 854–863.
10. Kushwah, R., Kaur, T., Dykes, C.L., Ravi Kumar, H., Kapoor, N. & Singh, O.P. 2020. A new knockdown resistance (kdr) mutation, F1534L, in the voltage-gated sodium channel of Aedes aegypti, co-occurring with F1534C, S989P and V1016G. Parasites & Vectors, 13(1), 327.
11. Lee, H. L. 1992. Aedes ovitrap and larval survey in several suburban communities in Selangor, Malaysia. Tropical Biomedicine, 9, 29-34.
12. McKenzie, B.A., Wilson, A. & Zohdy, S. 2019. Aedes albopictus is a competent vector of Zika virus: A meta-analysis. PloS One, 14(5), e0216794.
13. Ranson, H., Jensen, B., Vulule, J.M., Wang, X., Hemingway, J. & Collins, F.H. 2000. Identification of a point mutation in the voltage-gated sodium channel gene of Kenyan Anopheles gambiae associated with resistance to DDT and pyrethroids. Insect Molecular Biology, 9(5), 491–497.
14. Rocha, H., Paiva, M., Silva, N.M., de Araújo, A.P., Camacho, D., Moura, A., Gómez, L.F., Ayres, C. & Santos, M. 2015. Susceptibility profile of Aedes aegypti from Santiago Island, CaboVerde, to insecticides. Acta Tropica, 152, 66–73.
15. Rohani, A., Chu, W.L., Saadiyah, I., Lee, H.L. & Phang, S.M. 2001. Insecticide resistance status of Aedes albopictus and Aedes aegypti collected fromurban and rural areas in major towns of Malaysia. Tropical Biomedicine, 18, 29–39.
16. Rohani, A., Nazni, W.A., Bugor, H. & Lee, H.L. 1998. Evaluation of susceptibility of urban and rural Aedes albopictus to commonly used insecticides. Vector Journal, 4, 15-27.
17. Saingamsook, J., Saeung, A., Yanola, J., Lumjuan, N., Walton, C., & Somboon, P. 2017. A multiplex PCR for detection of knockdown resistance mutations, V1016G and F1534C, in pyrethroid-resistant Aedes aegypti. Parasites & Vectors, 10, 465.
18. Sayono, S., Hidayati, A.P., Fahri, S., Sumanto, D., Dharmana, E., Hadisaputro, S., Asih, P.B. & Syafruddin, D. 2016. Distribution of Voltage-Gated Sodium Channel (Nav) Alleles among the Aedes aegypti Populations in Central Java Province and Its Association with Resistance to Pyrethroid Insecticides. PloS One, 113(3), e0150577.
19. WHO (World Health Organization). 2016. Monitoring and managing insecticide rresistance in Aedes mosquito population. Interim guidance for entomologists, viewed 1 June 2021 https://apps.who.int/iris/bitsstream/handle/10665/204588/WHO_ZIKV_VC_16.1_engpdf?seuence=2.