Abstract and References
Transactions on Science and Technology Vol. 6, No. 4, 334 - 339, 2019

Electronic Nose detection of Ganoderma boninense Volatile Organic Compounds (VOCs) Using Direct Headspace Analysis

Li Wen Cheah; You Keng Goh; Kah Joo Goh; Maggie Tham; Sze Khai Wong

ABSTRACT
Electronic nose detection techniques have found wide applications in many fields including the medical and health sector, food industry, and also agriculture. Detection of volatile organic compounds (VOCs) emitted by Ganoderma boninense is increasingly explored as a reliable means of accurately diagnosing the presence of this important oil palm pathogen, facilitating management of the disease and preventing further spread in the field. For this purpose, we tested an electronic nose (‘zNOSETM 4200’) to detect G. boninense VOCs via direct headspace analysis from samples of trunk cores and inoculated oil palm ramets. We successfully identified five VOC markers (two for healthy tissue, three associated with G. boninense infection). Based on gas chromatography-mass spectrometry (GC-MS) of samples and analytical standards, one of the VOC markers for G. boninense-infected palms was positively identified as 2, 4-di-tert-butylphenol. These markers can be used in the next stage of optimization and field testing.

 KEYWORDS: Volatile organic compounds; direct headspace; early detection; basal stem rot; Ganoderma boninense



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