Abstract and References
Transactions on Science and Technology Vol. 5, No. 1, 46 - 52, 2018

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Survival and Pathogenicity of Monokaryotic and Dikaryotic Ganoderma boninense following three different Preservation Methods

Hun Jiat Tung, Cu Ean Ong, Yit Kheng Goh, You Keng Goh, Kah Joo Goh

ABSTRACT
Ganoderma boninense, is the major causal agent of basal stem rot (BSR) in oil palm, causing approximately RM 1.5 billion economic losses annually. Pure dikaryotic and monokaryotic G. boninense cultures are frequently used for epidemiology and aetiology studies of BSR. To obtain consistent and comparable inter-experiment results, maintaining and preservation of true-to-type pure cultures of monokaryotic and dikaryotic G. boninense are important. Three different pure fungal culture preservation techniques, namely serial sub-culturing, on slant overlaid with mineral oil, and submersion in water, were adopted to store four G. boninense isolates (two monokaryons and two dikaryons) for 12 months. Viability of G. boninense cultures preserved on agar slant overlaid with mineral oil and submersion in water, ranged from 50 to 89%. Our results also showed that one-year-old dikaryotic G. boninense isolates preserved in mineral oil and water had higher pathogenicity level compared to cultures from repeated sub-culturing. Both slant with mineral oil or in water preservation methods, which are less laborious, inexpensive, and simple, can be adopted for preserving pathogenic G. boninense.

KEYWORDS: Basidiomycete, Mineral Oil, Pathogenicity, Sub-culturing, Viability

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