Abstract and References
Transactions on Science and Technology Vol. 4, No. 3-3, 420 - 425, 2017

ISSR-PCR Fingerprinting of Kappaphycus and Eucheuma (Rhodophyta, Gigartinales) Seaweed Varieties From Sabah, Malaysia

Grace Joy Wei Lie Chin, Mohd Zarifi Mohamad, Salahaudin Maili, Wilson Thau Lym Yong, Kenneth Francis Rodrigues

ABSTRACT
Kappaphycus and Eucheuma seaweeds are commonly cultivated for carrageenan production in Sabah, Malaysia. Identification of the different varieties of Kappaphycus and Eucheuma seaweeds is important because certain seaweed varieties of the same species exhibit desired characteristics such as higher carrageenan content, faster growth rates and resistance to disease. The present study set forth to characterize Kappaphycus and Eucheuma seaweed varieties based on the genetic fingerprinting using inter-simple sequence repeat–polymerase chain reaction (ISSR-PCR) method. A total of eight ISSR primers were used to produce distinct and reproducible patterns of polymorphic bands. A total of 1494 bands of 180 to 4,600 bp were successfully amplified, of which 94.3% were polymorphic. The dendogram results showed a clear differentiation among the cultivar varieties of Kappaphycus seaweed only; however Eucheuma seaweed varieties have the same band profiles. Interestingly, 22 identical variety-specific ISSR bands were obtained from 30 seaweed samples and these can be applied as molecular tools for the identification of seaweed varieties. The data obtained from ISSR analysis can be used in breeding technology and various other applications for development of seaweed industries.

KEYWORDS: Eucheuma; genetic fingerprinting; inter-simple sequence repeat–polymerase chain reaction (ISSR-PCR); Kappaphycus; seaweeds.

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