Abstract and References
Transactions on Science and Technology Vol. 4, No. 3-3, 414 - 419, 2017

Antiproliferative Effect of Strobilanthes crispus on MCF-7 Cell Line

Norasyidah Gordani , Bo Eng Cheong, Peik Lin Teoh
ABSTRACT
 Despite the advancement of chemical biology and combinatorial chemistry, natural products remain a potent source of the anticancer drug development today. Strobilanthes crispus (S. crispus) which is from the Acanthaceae family has been traditionally used as medicine in several countries and reported to have anticancer, antioxidant, free radical scavenging, antidiabetic, antimicrobial, wound healing and antiulcerogenic activities. Thus, the aim of this study was to investigate the antiproliferative properties of S. crispus towards breast cancer cell line. The chemical compounds were extracted from various parts of the plant using methanol then followed by liquid-liquid partition. The antiproliferative effects of these extracts were tested on MCF-7. Among the extracts, only five showed inhibition of cell proliferation in MCF-7. The best antiproliferative activity was observed in stem ethyl acetate and leaf water extract with the IC50 value of 38 µg/ml and 23 µg/ml respectively. However, the IC50 values for stem chloroform, leaf methanol and leaf chloroform extracts were at the range of 70-90 µg/ml. Treatment with S. crispus extracts also caused morphological changes on MCF-7 cells. Chromatin condensation and peripheral aggregation of nuclear chromatin were observed in the treated cells. However, further investigation is needed to understand its underlying mechanisms.
KEYWORDS: Strobilanthes crispus; anticancer property; Breast cancer; MCF-7; antiproliferation
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