Abstract and References
Transactions on Science and Technology Vol. 4, No. 3-3, 384 - 390, 2017

Phytochemical Constituents and Antihyperglycemic Activity of Lygodium microphyllum Against Alloxan Induced Diabetic Rats

Dg Syahidah Nadiah binti Abdull Majid, Mohammad Iqbal
ABSTRACT
 Diabetes mellitus (DM) is a complex, chronic illness which requires continuous medical care as well as with multifactorial risk-reduction strategies beyond glycaemic control. Approximately 70% of the world’s population use traditional medicines derived from medicinal plants. The present study was designed to evaluate the phytochemical constituents and antihyperglycemic activity of Lygodium microphyllum. The phytochemical screening of the plant showed the presence of flavonoids, alkaloids, tannins, and steroids. Total phenolic content of leaves was found to be 966.7 ± 0.03 mg/g (expressed as milligram gallic acid equivalent per gram of plant extract). Total flavonoid content of leaves was found to be 42.9 ± 0.01 mg/g (expressed as milligram catechin equivalent/g). Diabetes was induced by intravenous injection of alloxan monohydrate at a dose of 100 mg/kg body weight in rats.  Blood glucose along with oxidative stress markers in pancreatic homogenate was assayed. Pancreas was also examined by haematoxylin/eosin staining. Treatment of diabetic rats with L. microphyllum at the dose level of 300, 400, and 500 mg/kg body weight once a day for 14 days prevented induction in blood glucose and attenuated alloxan induced oxidative stress. The protection was further evident through decreased histopathological alteration in pancreas. As a conclusion, optimistically this study will contribute towards validation of the traditional use of L. microphyllum in the treatment of diabetes. This study may be helpful in the prevention of diabetes complication associated with oxidative stress.

 KEYWORDS: Diabetes; Lygodium microphyllum; Alloxan; phytochemical constituents; antihyperglycemic activity
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