Abstract and References
Transactions on Science and Technology Vol. 4, No. 3-3, 354 - 359, 2017

Identification of Bioactive Compounds, Quantitative Measurement of Phenolics and Flavonoids Content, and Radical Scavenging Activity of Lygodium circinnatum

Riana Binti Awang Saman, Ruzaidi Azli Mohd. Mokhtar, Mohammad Iqbal
ABSTRACT
 Studies have proven that oxidative stress plays a major contribution towards many diseases. The ability of antioxidants status to recover from certain diseases is shown to be important for the improvement of human’s health. Lygodium circinnatum (Lygodiaceae) is used by local Sabahan in treating various diseases. In this study, the presence of bioactive compounds in the aqueous extract of L. circinnatum were chemically tested and has resulted in the detection of these compounds; alkaloids, flavonoids, tannins, phenolics compound and glycosides. Total content of phenolics and flavonoids were determined by using quantitative measure. In-vitro antioxidant activity was investigated by the method for total antioxidant capacity, 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity using UV-VIS spectrophotometer. The content of phenolics was 31.84 + 0.24 mg/ml gallic acid equivalent and flavonoids were 63.5 + 1.67 mg/ml catechin equivalent. The IC50 value of L. circinnatum in DPPH assay was 143.76 µg/ml whereas IC50 of standard ascorbic acid was 39.43 µg/ml. The results obtained have shown such important phytochemical properties present in L. circinnatum and is expected to be beneficial in treating diseases where oxidative stress is implicated.
KEYWORDS: Oxidative stress; L. circinnatum; aqueous extraction; phenolics; flavonoids; radical scavenging activity
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