Antioxidants, Polyphenols and Marker Phytochemicals Content from Different Parts of Clinacanthus nutans (C.nutans)
Norliza Abdul Latiff , Chua Lee Suan, Siti Nor Azlina Abd Rashid, Noor Azwani Zainool
Clinacanthus nutans (C.nutans) or Belalai Gajah has become popular medicinal herb for Malaysian community due to worthy biological property. It has been used in widespread of food products such as tea and supplements. The present study was focussed the determination of phytochemical components of C.nutans of the three different plant parts consists of leaves, stem and plant mixture. The experiments deal with the assessment of antioxidant property, total phenolic content and marker phytochemicals (orientin and vitexin). Free radical scavenging activity based on DPPH and ABTS assays were carried out for the antioxidant property. Meanwhile, the phenol content and identification of marker phytochemical were measured by using Folin-Ciocalteu’s method and HPLC analysis respectively. Leaves samples exhibits stronger scavenger ability (DPPH, 953.22± 3.49a µg/ml and ABTS,1693.33±0.23a µg/ml) compared to other samples. The total phenols content and both marker phytochemical of orientin and vitexin were found higher in leaves among the samples. Stem indicated the least results among the parameters tested. The total phenolic content in the leaves was 125.83 ± 0(mg GAE/g) while orientin represented 0.1235, w/w% and vitexin represented 0.1421 w/w%, respectively. The present results indicated leaves from C.nutans have higher chemical property and could be preferable part to be used for further phytochemicals research.
KEYWORDS: Clinacanthus nutans; polyphenols; antioxidant property; vitexin; isovitexin
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