Abstract and References
Transactions on Science and Technology Vol. 4, No. 3-3, 330 - 335, 2017

Chemical Characterization and Biological Activities of Methanol Extract From Castanopsis megacarpa Seeds of Sarawak

Nadia Binti Mat, Zaini Bin Assim, Fasihuddin Badrudin Ahmad
ABSTRACT
 Castanopsis genus belongs to Fagaceae family consists of 120 species distributed throughout temperate and tropical forest of Asia. Castanopsis megacarpa can be found along river and hillside are widely distributed in Peninsular Malaysia and Borneo Island. C. megacarpa seeds were initially defatted using petroleum ether and then extracted using methanol. Non-polar and polar fractions of methanol extract were analysed on Gas Chromatography-Mass Spectrometry (GC-MS). Non-polar combined fractions (CFa to CFc) contained hydrocarbons and several siloxanes. GC-MS analysis on derivatised polar combined fractions (CFd to CFg) revealed several peaks which correspond to carbohydrates, alcohols and fatty acids. Methanol extract and CFg sample showed antioxidant activities are high with low EC50 values of 205.10 and 158.97 mg/L, respectively. Brine shrimp lethality test showed methanol extract and polar combined fractions (CFd-CFg) are toxic with LC50 < 100 mg/L.
KEYWORDS: Castanopsis megacarpa; Gas Chromatography-Mass Spectrometry (GC-MS); derivatisation; antioxidant; toxicity
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REFERENCES

Akinterinwa, O., Khankal, R. and Cirino, P.C. (2008). Metabolic engineering for bioproduction of sugar alcohols. Current Opinion in Biotechnology, 19(461), 461-467.

Bastidas, A.J.O. (2013). Chemical characterization by gc-ms and phytotoxic potential of non-polar and polar fractions of seeds of Dioteryx odorata (Aubl.) Willd. from Venezuelan regions. Quimica Nova, 36(4), 502-506.

Dolai, N., Karmakar, I., Kumar, R.B.S., Bala, A., Mazumder, U.K. and Haldar, P.K. (2012).  Antitumor potential of Castanopsis indica (Roxb. Ex Lindl.) A. DC. leaf extract against Ehrlich’s ascites carcinoma cell. Indian Journal of Experimental Biology, 50, 359-365.

Gamble, J.S. (1914). New Fagaceae from the Malay Peninsula. Bulletion of Miscellaneous Information (Royal Botanic Garden, Kew),  5, 177-181.

Gupta, R. and Dhingra, V. (2015). Plants and their toxic constituent’s forensic approach: a review. European Journal of Forensic Sciences, 2(2), 15-25.

Grembecka, M. (2015). Sugar alcohols—their role in the modern world of sweeteners: a review. European Food Research and Technology, 241, 1-14.

Kabubii, Z.N., Mbaria, J.M. and Mbaabu, P.M. (2015). Phytochemical composition and brine shrimp cytotoxicity effect of Rosmarinus officinalis. American Scientific Research Journal for Engineering, Technology and Sciences, 11(1), 127-135.

Khoomrung, S., Martinez, J. L., Tippmann, S., Ard, S. J., Buffing, M. F., Nicastro, R. and Nielsen, J. (2015). Expanded metabolite coverage of Saccharomyces cerevisiae extract through improved chloroform/methanol extraction and tert-butyldimethylsilyl derivatization. Analytical Chemistry Research, 6, 9-16.

Livesey, G. (2003). Health potential of polyol as sugar replacers with emphasis on low-glycaemic properties. Nutrition Research Reviews, 16, 163-191.

Mohan, A., Sagar, S., Priya, B. and Bhagyashri, T. (2013). Phytochemical screening, flavonoid content and antioxidant activity of ethanolic extract of Ceiba Pentandra. International Research Journal of Pharmacy, 4(2), 108-110.

Niranjan, K., Sathiyaseelan, V. and Jeyaseelan. E.C. (2013). Screening for anti-microbial and phyto chemical properties of different solvents extracts of leafs of Pongamia Pinnata. International Journal of Scientific and Research Publications, 3(1), 1-3.

Pan, P., Tang, Y., Sun, D., Jiang, J. and Song, X. (2014). Effect of ultrasonic-assisted pretreatment on hydrolysis and fermentation of acorn starch. Bioresources, 9(2), 2705-2716.

Seal, T. (2011). Determination of nutritive value, mineral contents and antioxidant activity of some wild edible plants from Meghalaya State, India. Asian Journal of Applied Science, 4(3), 238-246.

Schummer, C., Delhomme, O., Appenzeller, B. M. R., Wenning, R. and Millet, M. (2009). Comparison of MTBSTFA and BSTFA in derivatization reactions of polar compounds prior to GC/MS analysis. Talanta, 77, 1473-1482.