Abstract and References |
Transactions on Science and Technology Vol. 4, No. 3-3, 330 - 335, 2017 |
Chemical Characterization and Biological Activities of Methanol Extract From Castanopsis megacarpa Seeds of Sarawak |
Nadia Binti Mat, Zaini Bin Assim, Fasihuddin Badrudin Ahmad |
ABSTRACT Castanopsis genus belongs to Fagaceae family consists of 120 species distributed throughout temperate and tropical forest of Asia. Castanopsis megacarpa can be found along river and hillside are widely distributed in Peninsular Malaysia and Borneo Island. C. megacarpa seeds were initially defatted using petroleum ether and then extracted using methanol. Non-polar and polar fractions of methanol extract were analysed on Gas Chromatography-Mass Spectrometry (GC-MS). Non-polar combined fractions (CFa to CFc) contained hydrocarbons and several siloxanes. GC-MS analysis on derivatised polar combined fractions (CFd to CFg) revealed several peaks which correspond to carbohydrates, alcohols and fatty acids. Methanol extract and CFg sample showed antioxidant activities are high with low EC50 values of 205.10 and 158.97 mg/L, respectively. Brine shrimp lethality test showed methanol extract and polar combined fractions (CFd-CFg) are toxic with LC50 < 100 mg/L. KEYWORDS: Castanopsis megacarpa; Gas Chromatography-Mass Spectrometry (GC-MS); derivatisation; antioxidant; toxicity Download Full Text PDF |
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