Abstract and References
Transactions on Science and Technology Vol. 4, No. 3-3, 318 - 323, 2017

Microbial 16S rDNA Sequencing of Cultivable Bacteria Associated with Toxic Dinoflagellate, Pyrodinium bahamense var. compressum

Salley Venda Law, Kenneth Francis Rodrigues, Ann Anton, Grace Joy Wei Lie Chin
ABSTRACT
 Bacteria associated with toxic dinoflagellate, Pyrodinium bahamense var. compressum play an important role in paralytic shellfish toxin production during harmful algal bloom occurrences, which frequently spotted in the coastal waters of Sabah. The outbreak has caused a remarkable impact on economic losses to Sabah aquaculture industries, even worse, human illness and fatalities. The expression of PST during a toxic bloom remains elusive, furthermore studies on the bacterial diversity associated with Sabah P. bahamense are limited. Thus the study aimed to examine the cultivable bacteria diversity associated with P. bahamense through 16S rDNA sequence analysis. A total of 74 isolates were successfully obtained, which were predominated by the phyla proteobacteria. 41 of the isolates were gamma-proteobacteria; Alteromonas sp. (37), Marinobacter sp. (3), Methylophaga sp. (1), and 34 of the isolates were alpha-proteobacteria; Ruegeria sp. (29), Lutimaribacter sp. (5).The major bacteria strains identified were Alteromonas sp. and Ruegeria sp. which were previously reported as a possible PST-producing bacterium. The information from this study will provide important insights into the understanding the relationship between dinoflagellate-bacteria association as well as the role of these bacteria in toxin production.
KEYWORDS: 16S rDNA sequencing; bacteria; toxic dinoflagellate; Pyrodinium bahamense var. compressum; harmful algal bloom.
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