Growth and Biomass Production of Native Microalgae Chlorella sp., Chlamydomonas sp. and Scenedesmus sp. Cultivated in Palm Oil Mill Effluent (POME) at Different Cultivation Conditions
Harizah Bajunaid Hariz, Mohd Sobri Takriff
The main objective of this research was to study native microalgae Chlorella sp., Clamydomonas sp. and Scenedesmus sp. growth and biomass productivity at different source of nutrient, inoculum concentration and aeration factor. Agricultural wastewater, palm oil mill effluent (POME) was used as a nutrient source which was compared with Bold’s Basal Medium (BBM) by culturing the microalgae at different inoculum concentration in 1-Litre transparent vessel with 700ml of working volume. Fixed aeration rate, 1L/min was set for all with 14000 lux of light intensity at 25±2°C cultivation temperature. The results were also compared with the non-aerated cultures which only mixing was being provided for the microalgae growth. Based on the growth rate, 30% of inoculum shows the highest growth rate for all these three microalgae species Chlorella sp. (µmax =0.2712), Chamydomonas sp. (µmax =0.2547) and Scenedesmus sp. (µmax =0.1867) cultured in POME with air being supplied as the aeration factor. However, in BBM 30% of inoculum for Chlamydomonas sp. (µmax =0.5082) and Scenedesmus sp. (µmax =0.3402) manifest highest growth rate while Chlorella sp. does not exhibit significant effect at different inoculum concentration. The non-aerated culture condition shows 20% of inoculum for Chlamydomonas sp. (µmax =0.125) and Chlorella sp. (µmax =0.2052) cultures give the highest growth rate with POME. Similar results were obtained for Chlamydomonas sp. (µmax =0.1673) and Chlorella sp. (µmax =0.1855) cultured in BBM at non-aerated condition. Scenedesmus sp. shows the highest growth rate with 10% of inoculum at the non-aerated condition for both POME (µmax =0.1900) and BBM (µmax =0.1975). From the growth curves, the adaptation (lag phase) and exponential period of microalgae species were determined. Based on the results, all 3-species cultured in POME required 6 days for the lag phase and 14 days for the exponential phase which is doubled the period taken for BBM as the nutrient medium.
KEYWORDS: Microalgae, Wastewater, Palm Oil Mill Effluent (POME), Culture Condition, Nutrient Source
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