Preliminary evaluation of CETP inhibition from selected Garcinia species

Suraya Abdul Sani; Christopher Wiart; Teng Jin Khoo

Preliminary evaluation of CETP inhibition from selected Garcinia species

Suraya Abdul Sani, Christopher Wiart & Teng Jin Khoo

Keywords: Cholesteryl ester transfer protein; Cholesterol lowering agent; Folklore medicine; Garcinia atroviridis; Garcinia parvifolia

A b s t r a c t

Two types of Garcinia species which are Garcinia parvifolia and Garcinia atroviridis Griff ex T. Anders were selected and being labelled as UNMC 45L, UNMC 78T and UNMC 78T based on the folklore medicine ‘myths’ that claiming Garcinia species has the ability to be anti-cholesterol. All of these three plant parts were evaluated for therapeutic potential as CETP inhibitors by using CETP Inhibitor drug screening kit. Extraction of crude material from plants was performed via gradient maceration in hexane, ethyl acetate and ethanol. All of the extracts show significant inhibition towards CETP activity. Ethanol extracts of UNMC 45L shows greatest inhibition as the IC50 is 15.43 ± 0.4212 mg/ml followed by Hexane extract and Ethyl Acetate extracts of UNMC 78L which are 28.70 ± 1.320 mg/ml and 28.49 ± 1.126 mg/ml respectively. However, all of the extracts of UNMC 78T shows lowest inhibition towards CETP activity and it is assumed that more bioactive compound could be present in the leaves compare to twigs. The positive findings from this study suggest that Garcinia species was effective natural inhibitors towards CETP.

© Transactions on Science and Technology 2016

References

[1] Assmann, G., Schulte, H., Von Eckardstein, A. & Huang, Y. (1996). High-density lipoprotein cholesterol as a predictor of coronary heart disease risk. The PROCAM experience and pathophysiological implications for reverse cholesterol transport. Atherosclerosis, 124(Suppl), S11-20

[2] Amran, A. A., Zaiton, Z., Faizah, O. & Morat, P. (2009). Effetcs of Garcinia Atroviridis on serum profiles and artherosclerotic lesions in the aorta of guinea pigs fed a high cholesterol diet. Singapore Medical Journal, 50(3), 295-299.

[3] Davidson, M. H., Maki, K.., Umporowicz, D., Wheeler, A., Rittershaus, C. & Ryan U. (2003). The safety and immunogenicity of a CETP vaccine in healthy adults. Atherosclerosis, 169(1), 113-120

[4] De Grooth, G. J., Kuivenhovenja, Stalenhoef, A. F., de Graaj, J., Zwinderman, A. H., Posma, J. L., van Tol, A. & Kastelein, J. J. (2002). Efficacy and safety of a novel cholestryl ester transfer protein inhibitor, JTT-705, in humans: a randomized phase II dose-response study. Circulation, 105, 2159-2165

[5] Garber, K. (1999). Waiting for cancer vaccines. Mod drug discovery, Pg 2-67

[6] Jabit, M. L., Wahyuni, F. S, Khalid, R., Israf, D. A., Shaari, K., Lajis, N. H. & Stanslas, J. (2009). Cytotoxicity and nitric oxide inhibitory activities of methanol extracts of Garcinia species. Pharmaceutical Biology, 47, 1019-1026

[7] Jena, B. S., Jayaprakasha, G. K., Singh, R. P. & Sakariah, K.K. (2002). Chemistry and Biochemistry of (-)-hydroxycitric acid from Garcinia. J Agric food Chem, 50, 10-22

[8] Johnson, T. (1999), CRC Ethnobotany Desk Reference. CRC Press, pg 357-360.

[9] Kannel, W. B. & Sytkowski, P.A. (1987). Atherosclerosis risk factors. Pharmacol Ther, 32(3), 207-35

[10] Krishnamurthy, K. & Sapna, V. (2008). Garcinia. In. Parthasarathy, V., Chempakam, B. & Zachariah, T. (Eds.). Chemistry of Spices. Illustrated ed., CABI.

[11] Lagrost, L. (1994). Regulation of cholesteryl ester transfer protein (CETP) activity: Review in vitro and in vivo studies. Biochem. Biophys Acta, 1215, 209-236

[12] Mackeen, M. M., Ali, A. M., Lajis, N. H., Kawazu, K., Hassan, Z., Amran, M., Habsah, M., Mooi, L.Y. & Mohamed, S. M. (2000). Antimicrobial, antioxidant, antitumour-promoting and cytotoxic activities of different plant part extracts of Garcinia Atroviridis griff ex T. anders. J. Ethnoparmacol, 72, 395-402

[13] Mackeen, M. M., Ali, A. M., Lajis, N. H., Kawazu, K., Kikuzaki, H. & Nakatani, N. (2002). Antifungal Garcinia Acid Esters from the Fruits of Garcinia Atroviridis. Z Naturforsch (C), 57, 291-295.

[14] National Heart Association of Malaysia (2010). Knowing your heart risks. http://www.malaysianheart.org/article.php?aid=598 (Accessed: 16 July 2011).

[15] Nazre, M., Clyde, M. & Latiff, A. (2007). Phylogenetic relationship of locally cultivated Garcinia species with some wild relatives. Malaysian Applied Biology, 36(1), 31-40

[16] PFIZER (13 February 2006): Drug development pipeline. Company Communication, 1, 1-15

[17] Rittershaus, C. W., Miller, D. P., Thomas, L. J., Picard, M. D., Honan, C. M., Emmett, C. D., Pettey, C. L., Adari, H., Hammond, R. A., Beattie, D. T., Callow, A. D., Marsh, H. C. & Ryan, U. S. (2000). Vaccine-induced antibodies inhibit CETP activity in Vivo and reduce Aortic Lesions in a Rabbit Model of Atherosclerosis. Arteriosclerosis, Thrombosis and Vascular Biology, 20, 2106-2112.

[18] Ritsch, A., Auer, B., Foger, B., Schwarz, S. & Patsch, J. (1993). Polyclonal antibody-based immunoradiometric assay for quantification of cholesteryl ester transfer protein. Journal of Lipid Research, 34, 673-679

[19] Stary, H. C., Chandler, A. B., Dinsmore, R. E., Fuster, V., Glagov, S., Insull, W. Jr., Rosenfeld, M. E., Schwartz, C. J., Wagner, W. D. & Wissler, R. W. (1995). A definition of advanced types of atherosclerotic lesions and a histological classification of atherosclerosis – A report from the Committee on Vascular Lesions of the Council on Artheriosclerosis, American Heart Association. Circulation, 92(5), 1355-1374

[20] Tall, A. R. (1993). Plasma cholesterol ester transfer protein. J. Lipid Res., 34, 1255-1274

[21] Wiart, C. (2006a). Medicinal plants of the Asia-Pacific drugs for the future? World Scientific Publishing CO. Pte.Ltd.

[22] Wiart, C. (2006b) Medicinal plant classified in the family Icacinaceae, Medicinal plants of Asia and the pacific. Illustrated ed., CRC Press, pg 434-640.